食品中乳酸菌定量检测方法研究进展

Research Progress on Enumeration Methods of Lactic Acid Bacteria in Food

目前,乳酸菌发酵剂因其益生作用在乳制品生产中广泛应用。由于乳酸菌活菌数量是重要的指标,因此对乳酸菌活菌的定量检测已然成为产品质量和功能评价的一项重要工作。本文就食品中乳酸菌的定量检测方法展开综述,详细介绍了平板菌落计数法、流式细胞术、荧光定量PCR法的检测原理、关键影响因素和研究进展,重点阐述了PMA结合荧光定量PCR法的优势和技术难点以及数字PCR法的前景和可行性,为推动食品中乳酸菌定量检测技术的进步提供了参考。

Lactic acid bacteria are commonly used as probiotic,and often associated with food production and preservation. Therefore,the enumeration of viable lactic acid bacteria cells,as the important index of products has become an important task for the quality control and functional evaluation of its related products. In this paper,the latest research progress on the detection methods of viable lactic acid bacteria cells at home and abroad was reviewed. The working mechanism,advantages and disadvantages of each method were analyzed,which was focus on the PMA combined qPCR method as well as the digital PCR method. The existing problems in this research field were pointed out and the follow-up research trend were forecasted. This paper provided a reference for promoting the progress on enumeration methods of viable lactic acid bacteria cells in food products.