林蛙卵油的提取、成分分析及抗氧化活性研究

Extraction,Component Analysis and Antioxidant Activity of Rana chensinensis Egg Oil

以林蛙卵为原料,采用超临界CO2提取法提取林蛙卵中的林蛙卵油,研究其成分含量和抗氧化活性。采用气相色谱-质谱法(GC-MS)联用对林蛙卵油的成分进行检测分析,确定成分及含量,并进行DPPH自由基清除能力试验、羟基自由基清除试验和超氧阴离子自由基清除试验确定林蛙卵油的抗氧化能力。通过单因素实验和正交试验确定最佳提取工艺为提取压力35 MPa,提取温度60℃,CO2流量10 L/h,提取时间150 min,此时林蛙卵油的提取率为34.26%。GC-MS结果显示林蛙卵油中含有34种脂肪酸,其中饱和脂肪酸和不饱和脂肪酸相对含量分别为31.95%和68.05%。体外抗氧化试验结果表明林蛙卵油对DPPH自由基的IC50值为0.897 mg/mL,对羟基自由基的IC50值为1.392 mg/mL,对超氧阴离子的IC50值为1.687 mg/mL。林蛙卵油具有较强的体外抗氧化活性,在天然抗氧化剂和保健食品中有一定的开发利用价值。

The egg oil of Rana chensinensis was extracted by supercritical CO2 extraction from the eggs of Rana chensinensis,the component content and antioxidant activity of the Rana chensinensis egg oil were researched.The components and the contents of Rana chensinensis egg oil were analyzed and detected used the method of gas chromatography-mass spectrometry(GC-MS).DPPH radical scavenging ability test,hydroxyl radical scavenging test and superoxide anion free radical scavenging test were carried out for determining the antioxdant capactity of Rana chensinensis egg oil.The optimum extraction conditions were determined by single factor test and orthogonal test were as follows:Extraction pressure 35 MPa,extraction temperature 60℃,CO2 flow rate 10 L/h and extraction time 150 min.The extraction rate of Rana chensinensis egg oil was 34.26%.The results of GC-MS showed that there were 34 fatty acids in the frog egg oil,the relative contents of saturated fatty acids and unsaturated fatty acids were 31.95%and 68.05%,respectively.The results of in vitro antioxidant test suggested that the IC50 value of Rana esculenta egg oil to DPPH free radical was 0.897 mg/mL,the IC50 value for hydroxyl radicals was 1.392 mg/mL,and the IC50value of superoxide anion was 1.687 mg/mL.The frog egg oil had effective activity in vitro antioxidant experiment and showed certain development and utilization value in natural antioxidants and health foods.