鼠李糖乳杆菌LR-ZB1107-01的安全性评价及其益生特性初步研究

Assessment of Safety and Probiotic Characteristics of Lactobacillus rhamnosus ZB1107-01

从广州1月龄婴儿粪便中分离得一株乳酸菌,经鉴定为鼠李糖乳杆菌(Lactobacillus rhamnosus ZB1107-01),通过对该菌的溶血性、抗生素敏感性和动物经口急性毒理等安全性评价,并研究标准菌株与其对致病菌抑菌性、胃肠道液耐受性、肠道黏附性、疏水性和自聚力。结果表明:此菌无溶血性;对四环素、红霉素、氨基西林和卡那霉素等4种抗生素表现高敏感;小鼠经口急性毒理无毒,确定该菌株为食用安全菌。该菌可抑制金黄色葡萄球菌、大肠杆菌、单增李斯特菌的生长,抑菌效果与标准菌株ATCC7469相当。对人工模拟的胃肠道液有很好的耐受性,对pH2的人工胃液耐受性略强于标准菌株。黏附Caco-2细胞数与标准菌株相当。疏水性(48.6%)高于标准菌株(27.2%),自聚性与标准菌株相当。研究为鼠李糖乳杆菌LR-ZB1107-01在食品领域进行商业化应用提供理论依据。

Lactobacillus(LCA)was isolated from 1-month-old Guangzhou baby feces.The LCA strain was identified as Lactobacillus rhamnosus.Safety evaluation experiments were implemented including hemolytic activity,antibiotic susceptibility and animal acute toxicity tests.Probiotic performance was determined by measuring the survivability in gastrointestinal,the ability of resisting pathogens,intestinal adhesion,hydrophobicity and self-aggregation,which would be compared with standard strain ATCC7469.The results showed that hemolytic activity was negative.Tetracycline,erythromycin,ampicillin and kanamycin had a strong inhibitory effect on LR-ZB1107-01.LR-ZB1107-01 was proved as an edible safe strain according to the results of animal acute toxicity tests.LR-ZB107-01 could inhibit the growth of Staphylococcus aureus,Escherichia,Listeria monocytogenes,the inhibition ability was equal to ATCC7469.It performed well in tolerating the intestinal environment even better in pH2 artificial gastric juice than stand strain.The number of adherent Caco-2 cells was also comparable to the standard strain.Hydrophobicity(48.6%)was higher than standard strain(27.2%)while its self-aggregation ability was equal to ATCC7469.The research could provide reference for the commercial application of LR-ZB1107-01 in the food field.