响应面法优化固相酸水解鲍鱼脏器多糖工艺

Optimization of Solid Phase Acid Hydrolysis of Polysaccharide from Abalone Viscera by Response Surface Methodology

为了研究鲍鱼脏器多糖固相酸(732#阳离子交换树脂)水解条件与多糖水解率之间的关系及其降解产物的分子结构,首先通过单因素实验确定树脂用量、水解时间和水解温度对鲍鱼脏器多糖水解率的影响,然后应用响应面法对鲍鱼脏器多糖水解条件进行设计优化,最后采用Sephadex G-25凝胶柱对鲍鱼脏器寡糖混合物进行分离纯化,并使用红外光谱分析对寡糖结构进行测定。结果表明:对多糖水解率的影响程度依次是水解温度、水解时间和树脂用量,最佳水解条件为:树脂用量40 g,水解时间3 h,水解温度70℃。在此条件下进行3次平行验证试验,鲍鱼脏器多糖水解率为80.69%,接近且略高于预测值,表明应用响应面法优化鲍鱼脏器多糖的水解条件是可行的。凝胶层析法纯化结果表明,鲍鱼脏器寡糖混合物中有两个组分,且第1组分占大多数。红外光谱分析表明,鲍鱼脏器寡糖为α-型糖,含有吡喃环。鲍鱼脏器多糖固相酸水解工艺研究,提高了鲍鱼脏器加工的经济效益,为鲍鱼脏器的高值化利用提供了参考。

In order to study relationship between the solid-phase acid(732-cation exchange resin)hydrolysis conditions of polysaccharide from abalone viscera and the hydrolytic rate of polysaccharide and the molecular structure of its degradation products.Firstly,the effects of resin dosage,hydrolysis time and hydrolysis temperature on the hydrolysis rate of abalone viscera polysaccharide were determined by single factor experiments.Then response surface methodology was used to optimize the hydrolysis conditions of abalone viscera polysaccharide.Finally,the abalone viscera oligosaccharide mixture was separated and purified by Sephadex G-25 gel column,and the structure of oligosaccharide was determined by infrared spectroscopy.The results showed that the degree of influence on the hydrolysis rate of polysaccharide was hydrolysis temperature,hydrolysis time and resin dosage in turn,and the optimal hydrolysis conditions were as follows:Resin dosage 40 g,hydrolysis time 3 h,hydrolysis temperature 70℃.Under three conditions of parallel verification test,the hydrolysis rate of abalone viscera polysaccharide was 80.69%,which was close to and slightly higher than the predicted value,indicating that it was feasible to optimize the hydrolysis conditions of abalone viscera polysaccharide by response surface methodology.Purification by gel chromatography showed that there were two components in the abalone viscera oligosaccharide mixture,and the first component accounted for the majority.Infrared spectroscopy analysis showed that the abalone viscera oligosaccharides wereα-type sugars and contained pyran ring.This study would improve economic efficiency of abalone viscera processing,and provide reference for its efficient utilization.