响应面法优化粗糙脉孢菌番茄红素的皂化提取工艺及其抗氧化活性

Optimization of Saponification Extraction Process of Lycopene from Neurospora crassa by Response Surface Methodology and Its Antioxidant Activities

本文研究了皂化反应结合有机溶剂对粗糙脉孢菌番茄红素提取的影响。通过单因素实验和响应面法对皂化提取条件进行了优化,包括皂化温度、皂化时间及NaOH浓度;同时以β-胡萝卜素、VC及VE为对照,测定了粗糙脉孢菌番茄红素对DPPH·、O-2·、·OH三种自由基的清除能力,并通过FRAP法测定其抗氧化能力。结果表明,最佳皂化工艺条件为:皂化温度58℃、皂化时间21 min、NaOH浓度0.06 mol/L,在此条件下番茄红素得率为(1.620±0.011)mg/g,比未皂化时提高了38.46%,抗氧化试验结果表明粗糙脉孢菌番茄红素具有良好的体外抗氧化活性,当其浓度为250μg/mL时对DPPH·及O-2·的清除活性最强,分别为86.43%、90.21%;当其浓度为1200μg/mL时,对·OH的最大清除率为85.83%。

In this work,the effect of saponification reaction combined with organic solvent on the extraction of lycopene from Neurospora crassa was studied.The optimal conditions of saponification extraction process were investigated by single factor experiments and the response surface methodology,including saponification temperature,saponification time and NaOH concentration.Meanwhile,compared withβ-carotene,VCand VE,the scavenging ability of DPPH radical,hydroxyl radical and superoxide anion radical and FRAP value were determined to evaluate the antioxidant activity of lycopene from Neurospora crassa.The results showed that the optimum saponification conditions were as follows:Saponification temperature of 58℃,saponification time of 21 min,NaOH concentration of 0.06 mol/L.Under this condition,the extraction yield of lycopene was up to(1.620±0.011)mg/g,which was 38.46%higher than that without saponification treatment.Besides,the lycopene extract revealed good antioxidant activities in vitro.The highest scavenging rates of DPPH·and O-2·(86.43%,90.21%,respectively)were achieved when the concentration of lycopene was 250μg/mL,while the lycopene of 1200μg/mL had the strongest antioxidant against·OH with 85.83%of scavenging rate.