碳硫质量比及碳源种类调控重力排水管道内甲烷及硫化氢排放特征

Emission Characteristics of Methane and Hydrogen Sulfide in Gravity Drainage Pipeline Controlled by m(C)/m(S)Ratio and Source Types

为了探究m(C)/m(S)及碳源种类对重力排水管网中释放的甲烷和H 2S的影响,构建模拟重力排水管网,通过更改进水m(C)/m(S)和碳源种类分析了甲烷,H 2S释放的释放特征及管网生物膜微生物的群落结构。结果表明m(C)/m(S)由4增加至8时,甲烷和H 2S的释放量由15.9 g和0.9 g下降至7.4 g和0.3 g,然而进一步升高m(C)/m(S)至16时,甲烷和H 2S的释放量显著提高。微生物种群分析揭示m(C)/m(S)提高促进了Desulfonema的相对丰度。碳源影响分析表明相较于乙酸盐,硫酸盐还原菌(SRB)更易利用丙酸盐作为电子供体生产H 2S。酶活性分析进一步揭示丙酸盐更易作为硫酸盐还原菌SRB的电子供体。

To explore the effects of m(C)/m(S)and carbon source types on methane and H 2S released from gravity drainage network,a simulated gravity drainage network was constructed.The characteristics of methane and H 2S release and the community structure of biofilm microorganisms in the network were analyzed by changing m(C)/m(S)and carbon source types.Results show that when m(C)/m(S)increased from 4 to 8,the release of methane and H 2S decreased from 15.9 g and 0.9 g to 7.4 g and 0.3 g,respectively.However,when m(C)/m(S)increased further to 16,the release of methane and H 2S increased significantly.Microbial population analysis revealed that m(C)/m(S)increased the relative abundance of Desulfonema,a sulfate-reducing bacterium(SRB).The analysis of carbon source effect showed that was more likely to use propionate as an electron donor to produce H 2S than to use acetate.Enzyme activity analysis further revealed that propionate was more likely to be the electronic donor of SRB.