儿童激素原发性肾病综合征抗增殖蛋白的研究

Analysis on the correlation of sterord-resistant nephrotic syndrome and prohibitin in children

目的:通过检测原发性肾病综合征(PNS)患儿血清抗增殖蛋白(PHB)水平,探讨其与激素耐药型肾病综合征(SRNS)的相关性,阐明PNS激素耐药的可能机制。方法:选取PNS患儿72例作为研究对象,分为29例SRNS及43例激素敏感型肾病综合征(SSNS)两组,以正常体检儿童60例作为对照组,应用Western Blot检测并比较其抗PHB水平。结果:对照组血清中PHB为阴性,SSNS组与SRNS组治疗前的抗PHB水平均高于对照组(P<0.000);SRNS组、SSNS组治疗前血清抗PHB水平比较,两组治疗前差异无统计学意义(P>0.05),SSNS组治疗后血清抗PHB水平明显低于治疗前(P<0.05),SRNS治疗后血清抗PHB水平与治疗前无明显差异(P>0.05)。结论:SRNS患儿血清PHB水平增高,提示其可能参与SRNS的激素耐药的发生发展过程。

Objective:(1) To detect the serum prohibitin protein(PHB) level in children with primary nephrotic syndrome(PNS) and analyze the correlation between PHB and sterord-resistant nephrotic syndrome(SRNS);(2)To expound the resistance mechanisms of SRNS. Methods: Serum PHB levels were determined by western blot in 72 children with PNS and 60 healthy children. Children with PNS were assigned into two groups, including 29 SRNS and 43 sterord-senstive nephrotic syndrome(SSNS). Results: Serum PHB was positive in children with SSNS and SRNS,however it was negative in the normal controls. Serum PHB levels were significantly higher in children with SRNS(0.237 5±0.108)or SSNS(0.207 6±0.125)than in normal controls(P<0.05). There was no significant changes in children with SSNS between SRNS.There was no significant changes before and after treatment in children with SRNS(0.195 0±0.025,P>0.05);however, serum PHB levels in children with SSNS was lower after four weeks or longer of treatment(0.000 5±0.002,P<0.05). Conclusion: Serum PHB levels were significantly increased in children with SRNS, suggesting that PHB might have a participation in the development of SRNS.