miRNA-21调控MACC1蛋白诱导宫颈癌细胞凋亡的研究

Study on the miRNA-21 regulates MACC1 protein induced apoptosis in cervical cancer cells

目的探讨miRNA-21对宫颈癌细胞生长和凋亡的影响。方法合成miRNA-21和对照微小RNA(miRNA)并转染到Hela宫颈癌细胞中。使用噻唑基蓝四唑溴化物(MTT)测定半胱天冬酶-3(caspase-3)活性,采用流式细胞术研究miRNA-21对Hela细胞生长和细胞凋亡的影响。合成靶向结肠癌转移相关基因-1(MACC1)的小干扰RNA,并将其与miRNA-21一起转染到Hela细胞中,分别通过免疫蛋白质印迹法和流式细胞术测量MACC1表达和细胞凋亡情况。结果miRNA-21转染到Hela细胞中抑制其生长,促进了其凋亡,同时活化的caspase-3表达水平降低。MACC1表达降低显著增加了miRNA-21诱导的Hela细胞凋亡。结论MACC1可能作为miRNA-21的作用靶点,参与宫颈癌细胞的凋亡行为调控。

Objective To investigate the effects of miRNA-21 on the growth and apoptosis of cervical cancer cells.Methods miRNA-21 and control MicroRNA(miRNA)were synthesized and transfected into Hela cervical cancer cells.The caspase-3 activity was investigated by thiazolyl blue tetrazolium bromide(MTT)assay,and the effect of miRNA-21 on Hela cell growth and apoptosis was studied by flow cytometry.Small interfering RNA of targeting MACC1 was synthesized and transfected into Hela cells together with miRNA-21.MACC1 expression and apoptosis were measured by Western blotting and flow cytometry,respectively.Results miRNA-21 was transfected into Hela cells to inhibit its growth and promote its apoptosis,while the expression level of activated caspase-3 was decreased.The decrease of MACC1 expression significantly increased the apoptosis of Hela cells induced by miRNA-21.Conclusion MACC1 may be a target of miRNA-21 and participate in the regulation of apoptosis in cervical cancer cells.