STIM1/TRPC1复合体在川崎病患儿冠状动脉损伤后修复中的调控作用

Regulatory role of STIM1/TRPC1 complex in the repair of coronary artery injury in children with Kawasaki disease

目的探讨基质交联分子1(stromal interaction molecule 1,STIM1)/瞬时受体电位通道1(transient receptor potential channel 1,TRPC1)复合体在川崎病免疫性冠状动脉损伤后修复中的调控作用。方法回顾性分析2015年1月至2018年6月温州医科大学附属新昌医院和温州医科大学附属第二医院收治的川崎病患儿73例,合并冠状动脉损伤(观察组)34例与未合并冠状动脉损伤(对照组)39例。采用Real-time PCR检测STIM1、TRPC1表达;采用免疫印迹法(WB法)检测STIM1、TRPC1表达;采用免疫共沉淀检测STIM1、TRPC1相互作用。结果观察组STIM1 Real-time PCR表达(0.54±0.12),低于对照组(0.87±0.19),差异有统计学意义(t=8.720,P<0.05)。观察组TRPC1 Real-time PCR表达(0.37±0.10),低于对照组(0.76±0.15),差异有统计学意义(t=12.866,P<0.05)。观察组STIM1表达相对灰度值(0.43±0.09),低于对照组(0.81±0.21),差异有统计学意义(t=9.790,P<0.05)。观察组TRPC1表达相对灰度值(0.30±0.08),低于对照组(0.67±0.14),差异有统计学意义(t=13.590,P<0.05)。结论STIM1/TRPC1复合体在川崎病冠状动脉损伤后修复中具有重要调控作用,呈低表达。

Objective To elucidate the regulatory role of matrix crosslinking molecule 1(STIM1)/transient receptor potential channel 1(TRPC1)complex in the repair of coronary artery injury in children with Kawasaki disease.Methods 73 children with Kawasaki disease treated in Xinchang Hospital and the Second Hospital Affiliated to Wenzhou Medical University from January 2015 to June 2018 were enrolled in the study,including 34 cases with coronary artery injury(observation group)and 39 cases with no coronary artery injury(control group).Real-time PCR was used to quantify STIM1 and TRPC1 genes,the expressions of STIM1 and TRPC1 were determined using Western blot(WB),and immunoprecipitation was used to identify the interaction between STIM1 and TRPC1.Results Real-time PCR showed that the copies of both STIM1 and TRPC1 genes were lower in the observation group than that in the control group(0.54±0.12 vs 0.87±0.19;0.37±0.10 vs 0.76±0.15),the differences were statistically significant(t=8.720,12.866,P<0.05).Meanwhile,the relative gray values of STIM1 and TRPC1 expression in the observation group were also significantly lower than those in the control group(0.43±0.09 vs 0.81±0.21;0.30±0.08 vs 0.67±0.14;t=9.790,13.590,P<0.05).Conclusions STIM1/TRPC1 complex plays an important regulatory role in the repair of coronary artery injury in children with Kawasaki disease in which both genes are less expressed.