摘要
目的探讨亚甲基四氢叶酸还原酶(MTHFR)基因C677T位点单核苷酸多态性与原因不明复发性流产(URSA)的发生之间的相关性。方法采用前瞻性病例对照研究,选取清远地区71例URSA患者与76例两次正常生育妇女作为对照,采用实时定量PCR分析技术检测MTHFR基因C677T位点单核苷酸多态性情况,并用χ^2检验分析URSA组与对照之间的C677T位点基因型的差异。结果 URSA组MTHFR(C677T)基因纯合突变型TT及等位基因T的频率均高于正常对照组。结论清远地区不明原因复发性流产的发生与MTHFR基因C677T位点纯合突变型TT存在相关性。
Objective:To investigate the correlation between single nucleotide polymorphism(SNP)at C677T locus of methylenetetrahydrofolate reductase(MTHFR)gene and unexplained recurrent abortion(URSA).Methods:A prospective case-control study was conducted.71 URSA patients and 76 normal births women in Qingyuan area were selected as controls.The SNP at C677T locus of MTHFR gene was detected by real-time quantitative PCR analysis,genotype differences at C677T locus between URSA group and control group were analyzed by χ^2 test.Results:The frequency of homozygous TT and T allele of MTHFR(C677T)gene in URSA group was higher than that in normal control.Conclusion:The occurrence of unexplained recurrent abortion in Qingyuan is correlated with homozygous TT at C677T locus of MTHFR gene.
作者
钟小烨
黎璐珊
陈瑞坚
符芳
雷婷樱
邓琼
张雯雯
杜坤
ZHONG Xiao-ye;LI Lu-shan;CHEN Rui-jian;FU Fang;LEI Ting-ying;DENG Qiong;ZHANG Wen-wen;DU Kun(Institute of Prenatal Diagnosis,Qingyuan Maternal and Child Health Care Hospital,Qingyuan 511500,Guangdong;Institute of Prenatal Diagnosis,Guangzhou Women And Children Medical Center,Guangzhou 510000,Affiliated To Guangzhou Medical University)
出处
《中国优生与遗传杂志》
2020年第2期144-146,共3页
Chinese Journal of Birth Health & Heredity
