胚胎培养液中sHLA-G表达水平与临床结局相关性的研究

Expression of s HLA-G in embryo culture medium and its impact on pregnancy outcome of IVF-ET

目的探讨胚胎培养液中可溶性的白细胞抗原-G(Soluble Human Leukocyte Antigen-G,sHLA-G)的表达与早期胚胎发育的关系及其对IVF-ET妊娠结局的影响。方法留取第3天移植及冷冻后的胚胎培养液,将微滴装入EP管置于-80冻存。ELISA法检测sHLA-G的表达水平。结果优质胚胎对应胚胎培养液中优质胚胎表达水平均明显高于非优质胚胎,差异有统计学意义(P<0.05)。妊娠组胚胎培养液中sHLA-G的表达水平显著高于非妊娠组,差异有统计学意义(P<0.05)。结论胚胎培养液中sHLA-G的表达水平与早期胚胎发育潜能及临床妊娠率呈现一定的相关性,可作为预测胚胎质量及妊娠结局的参考指标之一。

Objective:To investigate the relationship between soluble human leukocyte antigen-G(sHLA-G)expression in embryo culture medium and early embryonic development and the impact on pregnancy outcome of in vitro fertilization and embryo transfer(IVF-ET).Methods:Embryo culture mediums were obtained on the third day of transplantation and after freezing,ELISA was used to detect the expression level of sHLA-G.Results:The expression levels of sHLA-G in embryo culture medium of gradeⅠembryo and gradeⅡembryo were statistically significantly higher than those of grade Ⅲembryo(P<0.05).The expression level of sHLA-G in embryo culture medium in pregnancy group was statistically significantly higher than that in non-pregnancy group(P<0.05).Conclusion:The expression level of sHLA-G in embryo culture medium can be used as one of predictors of embryo quality and pregnancy outcome.