幽门螺杆菌感染性胃癌组织中cyclinD1、MMP-9的表达及其临床意义

Expression and Clinical Significance of CyclinD1 and Matrix Metalloproteinase-9 in Helicobacter Pylori Infected Gastric Carcinoma

目的:探讨幽门螺杆菌(HP)感染性胃癌组织中细胞周期蛋白D1(cyclinD1)、基质金属蛋白酶-9(MMP-9)的表达及其临床意义。方法:选取2016年12月到2018年6月期间在兰州大学第一医院接受治疗的胃癌患者80例,收集其手术切除的病理组织。采用C-14呼气试验和改良Giemsa染色检测患者HP感染的情况,采用免疫组化法检测胃癌组织中cyclinD1、MMP-9表达情况。分析HP感染、cyclinD1、MMP-9表达与胃癌患者临床病理特征的关系,并分析胃癌患者HP感染与cyclinD1、MMP-9表达的相关性。结果:80例胃癌患者HP感染阳性56例(70.00%),阴性24例(30.00%)。有淋巴结转移、浸润深度为T3+T4的胃癌患者的HP感染阳性率高于无淋巴结转移、浸润深度为T1+T2的胃癌患者(P<0.05)。80例胃癌患者cyclinD1阳性表达45例(56.25%),阴性表达35例(43.75%),MMP-9阳性表达65例(81.25%),阴性表达15例(18.75%),TNM临床分期为III+IV期、分化程度为低分化、有淋巴结转移、浸润深度为T3+T4的胃癌患者的cyclinD1、MMP-9阳性表达率明显高于TNM临床分期为I+II期、分化程度为中高分化、无淋巴结转移、浸润深度为T1+T2的胃癌患者(P<0.05)。HP感染阳性患者的cyclinD1阳性表达率和MMP-9阳性表达率均明显高于HP感染阴性患者(P<0.05)。Pearson相关分析显示,胃癌患者HP感染与cyclinD1、MMP-9表达均呈正相关(P<0.05)。结论:胃癌患者的HP感染情况与淋巴结转移、浸润深度有关,cyclinD1和MMP-9的表达与TNM临床分期、分化程度、淋巴结转移、浸润深度有关,且胃癌患者HP感染与cyclinD1、MMP-9表达均呈正相关。

Objective: To investigate the expression of cyclinD1 and matrix metalloproteinase-9(MMP-9) in helicobacter pylori(HP) infected gastric carcinoma and its clinical significance. Methods: 80 patients with gastric cancer who were treated in Lanzhou University First Hospital from December 2016 to June 2018 were selected and their pathological tissues were collected. C-14 breath test and modified Giemsa staining were used to detect the status of HP infection. Immunohistochemistry was used to detect the expression of cyclinD1 and MMP-9 in gastric cancer tissues. The relationship between HP infection and cyclinD1, MMP-9 expression and clinicopathological features of patients with gastric cancer was analyzed. The correlation between HP infection and cyclinD1, MMP-9 expression in patients with gastric cancer was analyzed. Results: In 80 patients with gastric cancer, 56 cases were positive for HP infection(70.00%) and 24 cases were negative(30.00%). The positive rate of HP infection in gastric cancer patients with lymph node metastasis and depth of invasion with T3+T4 was higher than that in gastric cancer patients without lymph node metastasis and depth of invasion with T1+T2(P<0.05). In 80 patients with gastric cancer, 45 cases were positive for cyclinD1 expression(56.25%), 35 cases were negative expression(43.75%), 65 cases were positive for MMP-9 expression(81.25%), 15 cases were negative expression(18.75%). The positive expression rates of cyclinD1 and MMP-9 in gastric cance patients with TNM stage for III+IV stage, low differentiation, lymph node metastasis and depth of invasion with T3+T4 were significantly higher than that of TNM stage for I+II stage, moderately high differentiation, no lymph node metastasis and depth of invasion with T1+T2(P<0.05). The positive rate of cyclinD1 and MMP-9 in HP positive group was significantly higher than that in HP negative group(P<0.05). Pearson correlation analysis showed that HP infection was positively correlated with cyclinD1 and MMP-9 expression in patients with gastric cancer(P<0.05). Conclusion: HP infection in patients with gastric cancer is related to lymph node metastasis and depth of invasion. The expression of cyclinD1 and MMP-9 is related to TNM clinical stage, differentiation, lymph node metastasis and depth of invasion.Moreover, HP infection is positively correlated with cyclinD1 and MMP-9 expression in gastric cancer patients.