基于二芳基四氮唑修饰短肽的光敏超分子水凝胶

Photosensitive supramolecular hydrogel based on biaryltetrazole modified with short peptide

本文报道了一种可对光照响应发生降解的超分子水凝胶,成胶因子为二芳基四氮唑修饰二肽(MeO-TetGA). MeO-Tet-GA在中性缓冲溶液中可发生自组装形成超分子水凝胶,并且该水凝胶具有良好的机械性能,动态储能模量G’在500 Pa以上.基于二芳基四氮唑与末端烯烃间的分子间光点击化学反应,我们可进一步在MeO-TetGA超分子水凝胶中修饰亲水性的苯基磺酸钠从而打破水凝胶内部的亲疏水平衡,进而诱发水凝胶内部纤维结构的破坏以及水凝胶的降解.此外,我们尝试了将MeO-Tet-GA超分子水凝胶应用于小鼠乳腺癌4T1细胞的三维培养,发现MeO-Tet-GA超分子水凝胶具有优良的生物相容性,在用于细胞三维培养3 d后不会造成明显的4T1细胞死亡.综上所述,由MeO-Tet-GA形成的超分子水凝胶是一种潜在的生物材料,在细胞长期三维培养以及通过光照调节细胞微环境进而研究细胞行为变化方面具有应用前景.

Here we report a photosensitive supramolecular hydrogel formed by MeO-Tet-GA. MeO-Tet-GA can selfassemble to form supramolecular hydrogel in neutral buffer. The formed hydrogel has good mechanical properties, and the dynamic energy storage modulus G′ is above 500 Pa. Based on the intermolecular photo-click reaction between biaryltetrazole and the terminal alkene, we could further modify the hydrogel with hydrophilic sodium 4-vinylbenzenesulfonate to disturb the hydrogel and induce the degradation of hydrogel. We also tried to use this supramolecular hydrogel for three-dimensional culture of mouse breast cancer 4T1 cells. We found that Me O-Tet-GA has excellent biocompatibility and did not cause significant 4T1 cell death after three days of culture. Overall, this work demonstrates that supramolecular hydrogel formed by Me O-Tet-GA holds great promise in long-term three-dimensional cell culture and in studying cell behaviors through photo-modulation of cell microenvironment.