飞蝗对两种禾本科植物挥发物的触角电位和行为反应

Electroantennogram and behavioral responses of Locusta migratoria to two graminaceous plant volatiles

【目的】检测飞蝗对2种禾本科植物挥发物--癸醛和芳樟醇的触角电位及行为反应,为了解飞蝗的嗅觉机制提供依据。【方法】首先,使用触角电位仪检测飞蝗4龄若虫对癸醛和芳樟醇4个剂量的触角电位反应。其次,使用嗅觉行为仪检测正常条件下4龄飞蝗对癸醛和芳樟醇不同剂量的行为反应。最后,采用嗅觉行为仪检测饥饿和饱食状态下的虫体对癸醛和芳樟醇的行为反应。【结果】4龄飞蝗对癸醛和芳樟醇的EAG值随着剂量增加呈先升高后降低的趋势,均在500μg达到最高值,且在该剂量下,雌雄虫之间的EAG值存在显著差异。正常条件下,1000μg芳樟醇对飞蝗雌雄虫均有显著的引诱作用,1500μg癸醛和芳樟醇显著引诱雄虫,500μg癸醛显著引诱雌虫。经饥饿处理后,雌虫被500μg和1000μg癸醛显著引诱,雄虫被250μg癸醛显著引诱,雌雄虫均被500μg的芳樟醇显著引诱。经过饱食处理后,250μg和500μg的芳樟醇显著引诱雌虫。芳樟醇对饥饿状态下飞蝗雄虫的引诱率均高于饱食状态,在500μg时,引诱率存在显著差异。【结论】飞蝗对这2种植物挥发物的触角电位反应存在剂量效应,雌雄虫触角电位在最佳剂量下存在显著差异。不同剂量的癸醛和芳樟醇可对飞蝗产生不同的引诱效果,饥饿状态下的虫体更易被芳樟醇所吸引。

[Objectives]To determine the electroantennogram(EAG)and behavioral responses of Locusta migratoria to 2 gramineous plant volatiles(decanal and linalool)and thereby contribute to understanding the olfactory mechanisms of the locusts.[Methods]The EAG responses of 4 th-instar nymphs to four different dosages of decanal and linalool were investigated using electroantennography and the behavioral responses of the locusts under normal conditions to four different dosages of decanal and linalool were determined with an olfactometer.The effects of decanal and linalool on the behavioral responses of food deprived and satiated locusts were also investigated.[Results]EAG response tended to first increase,then decrease,with increasing dosages of decanal and linalool up to a maximum of 500μg.There were significant differences in the EAG responses of female and male locusts to this dosage.Under normal conditions,1000μg of linalool was significantly attractive to both sexes.Male and female locusts were significantly attracted by 1500μg of decanal and linalool and 500μg of decanal,respectively.Food deprived,females were significantly attracted by 500 and 1000μg of decanal whereas food deprived males were significantly attracted by 250μg of decanal.Five hundred micrograms of linalool significantly attracted both sexes.Satiated female locusts were significantly attracted by 250 and 500μg of linalool.Linalool was more attractive to males that had been food-deprived than satiated males.Significantly more food-deprived locusts were attracted by 500μg of linalool than satiated locusts.[Conclusion]The EAG responses of the locusts to 2 plant volatiles were dose-dependent and differed significantly between the sexes at the optimum dose.Different dosages of decanal and linalool elicited different behavioral responses.Food-deprived locusts were more attracted by linalool than satiated locusts.