艾普拉唑钠对大鼠胃溃疡模型的影响

Effects of ilaprazole sodium on models of gastric ulcer in rats

目的:研究注射用艾普拉唑钠对幽门结扎和束水应激大鼠胃溃疡模型的治疗作用。方法:100只健康SD大鼠,随机分为模型对照组、艾普拉唑钠低、中、高剂量组(0.3,0.9和2.7 mg·kg^-1)和艾普拉唑片组(0.9 mg·kg^-1)。各给药组单次给予相应药液,模型对照组给予等量的溶剂。采用幽门结扎和束水应激法建立2种大鼠胃溃疡模型。通过测定胃酸总分泌量、胃液pH、胃蛋白酶活力、前胃和腺胃溃疡面积等指标以及组织病理学检查,与艾普拉唑肠溶片比较,探讨注射用艾普拉唑钠的药效作用。结果:艾普拉唑钠0.9和2.7 mg·kg^-1组胃液pH升高,胃溃疡面积、胃酸总分泌量、胃蛋白酶活力降低,各剂量组胃黏膜组织病理病变程度减轻,与模型对照组比差异具有统计学意义(P<0.05或P<0.01)。结论:艾普拉唑钠通过抑制胃酸分泌,对大鼠急性胃损伤具有治疗作用。

Objective: To study the therapeutic effects of ilaprazole sodium for injection on gastric ulcer models induced by pyloric ligation or water stress method in rats. Methods: One hundred SD rats were randomly divided into model control group, ilaprazole sodium groups(0.3, 0.9, 2.7 mg·kg^-1)and ilaprazole tablet group(0.9 mg·kg^-1). Each drug intervention group was given the corresponding drug solution once, and the model group was given equival amount of solvent.After the dosing, two gastric ulcer models were induced by ligated pylorus and water immersing in rats. The pharmacodynamic actions of ilaprazole sodium for injection were observed by determining total gastric acid output, gastric juice pH value, pepsin activity,forestomach or glandular stomach ulcer area and histopathological examination and compared with those of ilaprazole tablets. Results: Ilaprazole sodium groups(0.9, 2.7 mg·kg^-1) increased the pH value of gastric juice, and decreased gastric ulcer area,total gastric acid output, pepsin activity, and reduced the gastric mucosal histopathological lesions at each dose. Compared with the model control group, the differences were statistically significant(P<0.05 or P<0.01). Conclusion: Ilaprazole sodium has a therapeutic effect on acute gastric injury in rats by inhibiting gastric acid secretion.