云南省41对HIV-1双阳性夫妻的人口学及蛋白印迹带型特征分析

Analysis of demographic characteristics and blot bands pattern among 41HIV-1positive couples in Yunnan province

目的分析云南省艾滋病病毒(HIV)双阳性夫妻的人口学资料及蛋白印迹试验(western blot,WB)带型结果,了解其特征,为艾滋病在夫妻间传播的防治策略提供科学依据。方法收集云南省传染病医院2014-2016年HIV-1抗体检测阳性患者的样本和临床资料及配偶信息登记表,对双阳性夫妻的WB带型分布情况进行分析。结果41对HIV-1抗体阳性的夫妻,其中汉族占71.95%,其他少数民族占28.05%;年龄以≥51岁为主,占39.02%;其职业主要为农民(34.14%)和无业人员(29.27%)为主;传播途径以异性传播为主(67.07%);夫妻双方同1天被检测出占75.61%,间隔为1~2月、3~6月分别为19.51%和4.88%。各种条带在不同年龄及不同性别的条带检出差异无统计学意义(P>0.05);不同基因亚型中带型分布差异也无统计学意义,但从统计结果中可以看出gp41、P51、P55和P39在CRF01_AE亚型中阳性率最低,P55和P39在CRF07_BC亚型中阳性率最高,均为100.00%。结论云南省HIV-1双阳性夫妻以汉族、低文化水平和≥51岁人群为主。为减少HIV病毒在夫妻间传播,应加强宣传教育和行为干预,加大监测、检测,以减少HIV在夫妻同性传播。

Objective To analyze the demographic characteristics and western blot(WB)bands pattern among 41HIV-1positive couples in Yunnan province.Methods The specimens were collected from patients with positive HIV-1antibody in Yunnan Infectious Disease Specialist Hospital during 2014-2016and their clinical data were also retrieved.WB was performed on samples of 41HIV-1positive couples and the bands patterns were analyzed.Results Out of 41positive couples,Han nationality accounted for 71.95%,while other ethnic minorities accounted for 28.05%.39.02%of subjects were 51years or older.Most were farmers(34.14%)and unemployed(29.27%).Heterosexual transmission was the major way of transmission(67.07%).Couples were tested positive in the same day,over one month apart and over three months apart accounting for 75.61%,19.51%and 4.88%,respectively.There were no significant differences in the WB bands pattern of samples among cases with different age and gender(P>0.05).Even though the differences in band distribution of genotypes were not statistically significant,the positive rates of gp41,P51,P55and P39were the lowest in CRF01 AE subtype,while the positive rates of P55and P39 were the highest of 100.00%in CRF07_BC subtype.Conclusions In Yunnan,majority of HIV-1positive couples are those with Han nationality,low education level and age of 51or older.In order to prevent the heterosexual transmission,health education and behavioral intervention should be enhanced.