酶/酸水解毛叶木姜子中键合态香味成分的比较

Comparison of enzymatic and acid hydrolysis of bound aroma compounds in Litsea mollis Hemsl.

采用AmberliteXAD-2树脂吸附洗脱分离毛叶木姜子中的键合态香气物质前体,将分离得到的键合态香气物质前体在AR2000酶、果胶酶和酸3种条件下分别进行水解释放,采用气相色谱-质谱联用法(GC-MS)对水解后的键合态香气物质进行分离分析。结果表明:3种水解条件得到的键合态香气物质共有90种,主要为萜烯类和含氧萜类物质。其中,香叶醇在2种酶水解条件下均含量高,果胶酶水解条件下含量最高达21798.79μg/L;共34种香气物质具有明显香气特征,2种酶水解下最显著的香气特征为花香和甜香;酸水解条件下最显著的香气特征为花香。酶水解与酸水解条件下得到的键合态香气物质存在明显差异,果胶酶水解条件下所检出的香气物质种类多且含量较高。因此,果胶酶处理更有利于毛叶木姜子键合态香气成分的释放。

Bound aroma precursors from Litsea mollis Hemsl. were separated by Amberlite XAD-2 column chromatography and then hydrolyzed under AR2000 enzyme, pectinase and acid three different conditions to release bound aroma compounds, respectively. The obtained bound aroma compounds were analyzed by gas chromatography-mass spectrometry(GC-MS). The result showed that 90 bound aroma compounds were identified under three hydrolysis conditions, mainly including terpenes and oxygenated monoterpenes. Among them, geraniol was more abundant compound under two enzymatic hydrolysis conditions, its highest content of 21798.79 μg/L was obtained under the pectinase hydrolysis condition. A total of 34 aroma substances exhibited obvious aroma characteristics. And the most significant aroma characteristic was floral and sweet under two enzymatic hydrolysis conditions, and that was floral under the acid hydrolysis. There were obvious differences in the composition of bound aroma compounds under the enzymatic hydrolysis conditions and acid hydrolysis condition. The more kinds and high content of aroma compounds were detected under the pectinase hydrolysis condition. Therefor, pectinase hydrolysis is more conducive to release the bond aroma components of Litsea mollis Hemsl.