eHSP70、CRP在H型高血压辅助诊断及风险监测中的应用研究

Application of eHSP70 and CRP in the diagnosis and risk monitoring of H-type hypertension

目的初步探讨热休克蛋白70(eHSP70)、C反应蛋白(CRP)等指标在H型高血压辅助诊断及风险监测中的应用。方法分别收集体检健康者、非H型高血压及H型高血压患者血液标本,采用酶联免疫吸附试验(ELISA)检测血清中eHSP70的水平变化,全自动生化分析仪检测各组血清CRP、血脂等指标,全自动血液分析仪检测全血白细胞总数(WBC)。结果与健康对照组相比,非H型高血压组和H型高血压组eHSP70和CRP、WBC、三酰甘油均显著升高,差异有统计学意义(P<0.05),胆固醇差异无统计学意义(P>0.05)。H型高血压组较非H型高血压组eHSP70和CRP水平有显著升高,差异有统计学意义(P<0.05),高风险组(Hcy≥20μmol/L)较低风险组(10μmol/L≤Hcy<20μmol/L)eHSP70和CRP也有显著升高,差异有统计学意义(P<0.05)。结论H型高血压患者血清eHSP70和CRP均有显著升高,且与风险程度呈正相关关系,可将其作为H型高血压的临床辅助诊断和风险监测的实验室指标。

Objective To explore the application of heat shock protein 70(eHSP70)and C-reactive protein(CRP)in the auxiliary diagnosis and risk monitoring of H-type hypertension.Methods Blood samples of healthy people,non-H type hypertension and H type hypertension were collected,the level of eHSP70 in serum was detected by enzyme-linked immunosorbent assay(ELISA),serum CRP,blood lipid and other indexes were detected by automatic biochemical analyzer,and total WBC was detected by automatic blood analyzer.Results Compared with the healthy control group,eHSP70,CRP,WBC and triacylglycerol in the non-H type hypertension and H type hypertension groups were significantly higher,the difference was statistically significant(P<0.05),and the difference in cholesterol was not statistically significant.The level of eHSP70 and CRP in H-type hypertension group was significantly higher than that in non-H hypertension group,and the difference was statistically significant(P<0.05),and the level of eHSP70 and CRP in high-risk group(Hcy≥20μmol/L)was significantly higher than that in low-risk group(10μmol/L≤Hcy<20μmol/L),and the difference was statistically significant(P<0.05).Conclusion The serum levels of eHSP70 and CRP in patients with H-type hypertension significantly increased,which is positively correlated with the risk level.It could be used as a laboratory index for clinical auxiliary diagnosis and risk monitoring of H-type hypertension.