谷氨酰胺对阉割后断奶仔猪生长性能、血液参数及免疫应答的影响

Effects of glutamine on growth performance,blood parameters and immune response of weaned piglets after castration

本研究通过两个试验探讨了日粮添加谷氨酰胺对断奶仔猪去势后生长性能、血浆参数及脂多糖诱导的免疫应答的影响。在试验1中,48只断奶仔猪分别饲喂添加基础日粮和含有2%谷氨酰胺的日粮,共进行25d饲养试验。在试验2中,16只断奶仔猪在断奶后第14天注射了大肠杆菌K88+脂多糖。结果表明,在断奶后第25天,谷氨酰胺组体重显著高于对照组(P <0.05),同时谷氨酰胺组较对照组显著提高了15~25d和1~25d断奶仔猪的饲料效率(P <0.05)。与对照组相比,谷氨酰胺组显著提高了空肠和回肠肌层厚度(P <0.05)。在断奶后25d,谷氨酰胺组血浆碱性磷酸酶活性显著高于对照组(P <0.05)。在LPS刺激前的断奶后第14天,谷氨酰胺组较对照组显著降低了血浆促肾上腺皮质激素和皮质醇浓度(P <0.05)。当猪接受LPS刺激时,血浆促肾上腺皮质激素和皮质醇水平显著升高(P <0.05)。LPS刺激后,对照组直肠温度显著高于处理组(P <0.05)。断奶后第14天,LPS刺激前或后谷氨酰胺组血浆IgG浓度均显著高于对照组(P <0.05)。结论 :日粮中添加2%谷氨酰胺能缓解断奶仔猪去势相关的应激状态和炎症反应,提高仔猪免疫和生长、发育。

Two experiments were conducted to investigate the effects of dietary glutamine on growth performance,plasma parameters and lipopolysaccharide induced immune response of weaned piglets after castration.In trial 1,48 weaned piglets were fed a basal diet and a 2%glutamine diet for 25 days.In experiment 2,16 weaned piglets were injected with E.coli K88+LPS on day 14 after weaning.The results showed that on the 25 d after weaning,the weight of the glutamine group was significantly higher than that of the control group(P<0.05),and the feed efficiency of the 15~25 d and 1~25 d weaned piglets in the glutamine group was significantly higher than that of the control group(P<0.05).Compared with the control group,the glutamine group significantly increased the thickness of jejunum and ileum muscularis(P<0.05).At 25 d after weaning,serum alkaline phosphatase activity in glutamine group was significantly higher than that in control group(P<0.05).On the 14th day after weaning before LPS stimulation,plasma corticotrophin and cortisol concentrations in the glutamine group were significantly lower than those in the control group(P<0.05).Plasma levels of corticotropin and cortisol were significantly increased when pigs were treated with LPS(P<0.05).After LPS stimulation,rectal temperature in the control group was significantly higher than that in the treatment group(P<0.05).At the 14th day after weaning,before or after LPS stimulation,the plasma IgG concentration in the glutamine group was significantly higher than that in the control group(P<0.05).Conclusion:adding 2%glutamine to the diet can alleviate castration^related stress and inflammatory response of weaned piglets,and improve their immunity and growth ability.