银耳菌丝体多糖理化性质及其吸湿保湿性能测定

Identification of Physicochemical Properties,Hygroscopic and Moisturizing Properties of Polysaccharide from Mycelium of Tremella Fuciformis

目的:以银耳菌丝体为试验材料,制备银耳菌丝体多糖(PMT),研究其部分理化性质及吸湿保湿性能。方法:采用水提醇沉法制备PMT,硫酸-苯酚法测定其总糖含量,红外光谱法对其基团进行定性分析,ELSD-HPLC法测定其单糖组分,扫描电镜测定其表面形貌特征,称重法测定其吸湿与保湿性能。结果:PMT的总糖含量为81.52%,具有典型的多糖吸收峰,其单糖组成为Man∶Glc∶Gal=5.25∶2.82∶1.00,其表面不光滑,有许多呈花菜状凸起。在相对湿度43%、81%情况下,样品的吸湿率大小顺序为甘油>PMT>海藻酸钠。在干燥环境下,样品的保湿率大小顺序为PMT>海藻酸钠>甘油。结论:PMT是一种含有α-D-糖苷键的非淀粉类吡喃多糖,具有一定的吸湿保湿性能,可作为保湿因子在护肤品中具有开发应用潜力。

Objective:The polysaccharide from mycelium of Tremella fuciformis(PMT)was prepared,and its partial physical and chemical properties,moisture absorption and moisture retention were studied.Methods:PMT was extracted by water extraction and alcohol precipitation method.The total sugar content was determined by sulfuric acid-phenol method,the groups were qualitatively analyzed by IR,the monosaccharide components were determined by ELSD-HPLC method,the surface morphology was determined by scanning electron microscope,and the moisture absorption and moisturizing properties were measured by weighing method.Results:The total sugar content of PMT was 81.52%,which had a typical peak of polysaccharide absorption.PMT consisted of Man∶Glc∶Gal=5.25∶2.82∶1.00.The surface of PMT was not smooth,with many cauliflowers like protrusions.When the relative humidity is 43%and 81%,the order of moisture absorption rate was glycerin>PMT>sodium alginate.In the dry environment,the order of moisture retention rate was sodium PMT>alginate>glycerin.Conclusion:PMT is a kind of non starch pyran polysaccharide with α-D-glycosidic bond,which has certain moisture absorption and moisturizing retention properties,and could be used as a moisturizing factor in skin care products.