不同产地中华大蟾蜍浆液中蟾毒配基类成分含量对比研究

Contrast Study on Content of Bufogenin Constituents in Toad Venom of Bufo Bufo Gargarizans from Different Geographic Locations

目的调研不同产地中华大蟾蜍浆液有效成分含量差异,分析蟾酥药材的区域特征性。方法采用HPLC测定不同产地的蟾蜍浆液中7种主要活性成分含量,并采用层次聚类分析和雷达图探究样本的产地规律。结果不同产地蟾酥浆液中蟾毒配基类成分含量差异显著,华蟾酥毒基和酯蟾毒配基为华东地区蟾酥原料的主导成分,2种成分含量之和在6.95%~11.15%,而华中和西南地区2种成分含量之和在0.17%~4.17%;远华蟾蜍精和华蟾毒它灵为华中和西南地区的主导成分,2种成分含量之和在5.66%~11.37%,而华东地区2种成分含量之和在1.22%~2.26%。结论蟾酥原料质量主要受产地因素影响,该调研结果为完善蟾酥药材的质量控制提供参考。

OBJECTIVE To investigate the difference in the content of active ingredients in toad venom of Bufo bufo gargarizans from different geographic locations,and analyze the regional characteristics of the Bufonis Venenum.METHODS The contents of 7 main active compounds were determined by HPLC,and the geographic location discipline of the samples were analyzed by hierarchical cluster analysis and radar chart.RESULTS The contents of bufogenin in different geographic locations was significant different.The dominant ingredients of the Bufonis Venenum from Eastern were the cinobufagin and resibufogenin,the total content ranged from 6.95%to 11.15%,while the contents in Central and Southwestern China ranged from 0.17%to 4.17%.The dominant ingredients of the Bufonis Venenum from Central and Southwestern China were the telocinobufagin and cinobufotalin,the total content ranged from 5.66%to 11.37%,while the contents in Eastern ranged from 1.22%to 2.26%.CONCLUSION The quality of Bufonis Venenum raw materials is mainly affected by the factors of geographic location.The results provide an important reference for improving the quality control of Bufonis Venenum.