负载白藜芦醇的玉米醇溶蛋白-果胶纳米粒抗氧化活性及其对急性炎症小鼠的抗炎作用

Antioxidant Activity and Anti-inflammatory Effect on Acute Inflammatory Mice of Resveratrol-loaded Zein-pectin Nanoparticles

本文研究了负载白藜芦醇的玉米醇溶蛋白(zein)-果胶纳米颗粒(En-RES)对体外模拟消化后的抗氧化能力和LPS诱导小鼠的抗炎效果。采用反溶剂/静电沉积相结合的方法制备负载白藜芦醇的玉米醇溶蛋白(zein)/果胶核壳纳米粒。用模拟胃肠道消化的En-RES消化液处理H2O2诱导的Hep G2细胞氧化应激模型,检测丙二醛(MDA)和抗氧化酶的含量。72只小鼠随机分成正常组、脂多糖(LPS)模型组、游离白藜芦醇低、中、高浓度组、En-RES低、中、高浓度组,分别干预后取血浆测定白细胞介素-1α(IL-1α)、白细胞介素-1β(IL-1β)、白细胞介素-6 (IL-6)、白细胞介素-10 (IL-10)和一氧化氮(NO)水平。研究结果表明,与游离白藜芦醇(RES)消化液相比,En-RES消化液处理氧化应激的Hep G2细胞后,细胞内的MDA含量降低49.07%,超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性分别提高35.27%、67.22%和13.15%(p<0.05)。小鼠急性炎症实验中,与RES高浓度处理组相比,En-RES高浓度组中小鼠血浆IL-1α、IL-1β、IL-6和NO水平分别下降62.73%、44.91%、52.86%和55.47%,IL-10水平提高了66.49%(p<0.05)。负载白藜芦醇的zein-果胶纳米颗粒提高了白藜芦醇在体外的抗氧化能力和体内的抗炎能力。

The anti-oxidative ability after simulated digestion in vitro and the anti-inflammatory effects of LPS-induced mice of resveratrol-loaded zein-pectin nanoparticles(En-RES) were studied. An antisolvent/electrostatic deposition method was used to prepare En-RES. The H2O2-induced oxidative stress model was treated with En-RES digestive fluid after simulated gastrointestinal digestion, and the contents of malondialdehyde(MDA) and antioxidant enzymes were measured. The 72 mice were randomly divided into the normal group, lipopolysaccharide(LPS) model group, free resveratrol groups(low, medium and high concentrations) and En-RES groups(low, medium and high concentrations). The plasmas were collected for determination of interleukin-1α(IL-1α), interleukin-1β(IL-1β), interleukin-6(IL-6), interleukin-10(IL-10) and nitric oxide(NO) level. The results showed that, when compared with free resveratrol(RES) digestion fluid treated oxidative stress reacted HepG2 cells, the intracellular MDA content of the cells treated with En-RES digestion fluid decreased by 49.07%, and superoxide dismutase(SOD), glutathione peroxidase(GSH-Px) and catalase(CAT) activities were increased by 35.27%, 67.22% and 13.15%, respectively(p<0.05). In the mouse acute inflammation experiment, compared with the RES high concentration treatment group, the plasma levels of IL-1α, IL-1β, IL-6 and NO in the high concentration group of En-RES decreased by 62.73%, 44.91%, 52.86% and 55.47%, respectively, and IL-10 levels were increased by 66.49%(p<0.05). This study showed that resveratrol-loaded zein-pectin nanoparticles could improve the antioxidant capacity in vitro and anti-inflammatory capacity in vivo of resveratrol.