不同菌体肽聚糖对产气荚膜梭菌芽孢萌发的诱导作用

Induction of Germination of Clostridium perfringens Spores by Different Bacterial Peptidoglycans

为了研究不同菌体肽聚糖对芽孢萌发的影响,本研究分别提取了产气荚膜梭菌(Clostridium perfringens,C. perfringens)、枯草芽孢杆菌(Bacillus subtilis,B. subtilis)及金黄色葡萄球菌(Staphylococcus aureus,S. aureus)细胞壁肽聚糖,以C. perfringens芽孢为研究对象,按照1:10、1:100、1:1000比例分别加入提取的三种菌体肽聚糖,并以芽孢浑浊度(OD600)、芽孢折光性、2,6-吡啶二羧酸(DPA)的释放量、热抗性损失等指标比较不同菌体肽聚糖对C.perfringens芽孢萌发的效果。结果显示,按照一定百分比分别加入三种菌体肽聚糖,1:10比例添加C. perfringens营养体肽聚糖诱导C. perfringens芽孢萌发效果最佳,在60 min后,其芽孢浑浊度(OD600)下降了31.71%,DPA释放率上升可达到38.29%;1:10比例添加B. subtilis营养体肽聚糖诱导C. perfringens芽孢萌发效果次之,其芽孢浑浊度(OD600)下降了24.92%,DPA释放率上升可达到30.84%;而不同比例S. aureus营养体肽聚糖对C. perfringens芽孢萌发均无显著影响。本研究可为进一步阐明肽聚糖诱导芽孢萌发的机制提供重要信息。

In order to study the effect of different bacterial peptidoglycans on spore germination, cell wall peptidoglycans were extracted from Clostridium perfringens(C. perfringens), Bacillus subtilis(B. subtilis) and Staphylococcus aureus(S. aureus). Using C. perfringens spores as the research object, the three kinds of extracted bacterial peptidoglycans were added at the ratios of 1:10, 1:100, 1:1000, respectively. Based on the spore turbidity(optical density at 600 nm, OD600), spore refractive index, amount of released pyridine-2,6-dicarboxylic acid(dipicolinic acid [DPA]), loss of heat resistance and other indicators, the effects of different bacterial peptidoglycans on the germination of C. perfringens spores were compared. The results showed that among the three kinds of bacterial peptidoglycans added at a certain percentage, the addition of C. perfringens peptidoglycan at a ratio of 1:10 induced the greatest extent of germination of C. perfringens spores. After 60 min, the OD600 decreased by 31.71%, while the DPA release rate increased to 38.29%. The addition of B. subtilis vegetative peptidoglycan at a 1:10 ratio induced the germination of C. perfringens spores to the second greatest extent, with a decrease of OD600 by 24.92%, and the increase of DPA release rate to 30.84%. However, the addition of S. aureus vegetative peptidoglycan at different ratios affected insignificantly the germination of C. perfringens spores. The results of this study can provide important information for further elucidation of the mechanism underlying the germination of peptidoglycan-induced spores.