体外模拟胃肠消化对西瓜和苹果抗氧化活性的影响

The effect of simulated digestion in vitro on antioxidantactivity of watermelon and apple

通过体外模拟胃肠消化试验,本研究对西瓜和苹果果汁体外胃肠模拟消化前后抗氧化活性的变化进行了分析与比较.结果显示,在同等消化条件下,西瓜模拟胃肠消化的过程中,在0.5~1 h内自由基清除率逐渐升高,约在1 h达到峰值,随后清除率逐渐下降并呈现稳定趋势;西瓜肠消化后铁离子还原能力以及DPPH自由基清除率均低于胃消化;西瓜胃消化后亚硝酸根清除率与肠消化后无明显差异;西瓜胃消化后羟自由基消除率低于肠消化.而苹果肠消化后,DPPH自由基清除率和亚硝酸根均大于胃消化;但羟基清除率以及铁离子还原能力却小于胃消化.研究表明,西瓜和苹果外模拟胃肠消化后,抗氧化活性均有所升高.这为西瓜和苹果的体内代谢研究及其资源进一步开发利用提供了相应的科学依据.

The juice of watermelon and apple were treated by simulating gastrointestinal digestion in vitro and the changes of antioxidant activity were compared and analyzed.The results showed that under the same digestive conditions,the free radical scavenging rate increased within 0.5~1 h of digestion,reached the maximum value after digestion for about 1 h,and then decreased gradually while the tend is to be stable.The DPPH free radical scavenging rate and the reduction capacity of iron ions were lower than those of gastrointestinal digestion.The scavenging rate of nitrite in watermelon have no difference between gastrointestinal digestion and intestinal digestion,while the scavenging rate of hydroxyl radical in watermelon intestine was lower than that of gastrointestinal digestion.The DPPH free radical scavenging rate and nitrite scavenging rate were higher than those of gastrointestinal digestion,but the reduction ability of iron ions and hydroxyl scavenging rate were lower than those of gastrointestinal digestion.Studies have shown that the antioxidant activity of watermelon and apple increased after simulated gastrointestinal digestion.The results will provide scientific basis for the research on the metabolism of watermelon and apple and the further development and utilization of their resources.