拟南芥6个突变体热敏感性差异的比较分析

Comparison of Thermo-sensitivity among Six Mutants of Arabidopsis thaliana

为了解拟南芥(Arabidopsis thaliana)热敏感突变体的热敏感性,对6个常用的拟南芥热敏感突变体hot1、apx2、fes1a、hsfa7a、hop1-2-3和hsp70-15进行了比较分析。结果表明,6个突变体的热敏感性均高于野生型,但他们之间的热敏感性有显著差异,45℃极度高温下90 min,hot1的白化死亡率最高,处理105 min后,fes1a也出现高比率的白化死亡,处理135 min后,apx2、hsfa7a和hop1-2-3表现出几乎相同的损伤现象,热损伤均比hsp70-15严重。因此,6种突变体的热敏感性依次为hot1>fes1a>apx2、hsfa7a、hop1-2-3>hsp70-15。

In order to understand the thermo-sensitivity of heat sensitive mutants of Arabidopsis thaliana,the thermo-sensitivities were compared among six mutants,including hsp70-15,apx2,hop1-2-3,hsfa7a,fes1a and hot1.The results showed that the thermo-sensitivities of six mutants were higher than that of WT,but there were significant difference among them.The 8-day-old seedlings were adapted at 38℃for 2 h at first,then they were under heat stress at 45℃.Seedlings of mutant hot1 showed albinism with high mortality under 45℃for 90 min.Seedlings of mutant fes1a also suffered high death rate under 45℃for 105 min.Further,seedlings of mutants apx2,hsfa7a and hop1-2-3 showed almost identical injury phenomenon under 45℃for 135 min,their heat injury were more severe than that of mutant hsp70-15.Therefore,the thermo-sensitivities of six mutants were in the order of hot1>fes1a>apx2,hsfa7a,hop1-2-3>hsp70-15.