牛大力组织培养技术研究

Research on Tissue Culture Technology of Millettia specisoa

本试验通过组织培养手段,以牛大力种子为材料,对外植体诱导及无菌组织培养进行了尝试,以期获得牛大力外植体诱导、增殖和生根的适宜培养基和培养条件。结果表明,消毒方式为75%酒精消毒30 s+0.1%氯化汞消毒15 min,外植体诱导培养基为改良1/2 MS+蔗糖15 g/L+琼脂6 g/L+6-BA 0.4 mg/L+NAA 0.15 mg/L+益培灵0.20 g/L,增殖培养基为改良MS+蔗糖30 g/L+琼脂6 g/L+6-BA 0.6 mg/L+NAA 0.20 mg/L+益培灵0.10 g/L,生根培养基为改良1/2 MS+蔗糖15 g/L+琼脂6 g/L+IBA 1.5 mg/L+ABT 11.5 mg/L+益培灵0.05 g/L,较适宜牛大力组织培养。

In this experiment,tissue culture was carried out with Millettia specisoa Champ.seeds as the material,explant induction and aseptic tissue culture were attempted,in order to obtain the suitable culture medium and culture conditions for explants induction,multiplication and rooting of Millettia specisoa.The results showed that,the disinfection method was 75% alcohol disinfection for 30 s+0.1% HgCl2 disinfection for 15 min,the explant induction medium was improved 1/2 MS+sucrose 15 g/L+AGAR 6 g/L+6-BA 0.4 mg/L+NAA 0.15 mg/L+Yipeiling 0.20 g/L,and the multiplication medium was improved MS+sucrose 30 g/L+AGAR 6 g/L+6-BA 0.6 mg/L+NAA 0.20 mg/L+Yipeiling 0.10 g/L,the rooting medium was improved 1/2 MS+sucrose 15 g/L+AGAR 6 g/L+IBA 1.5 mg/L+ABT 11.5 mg/L+Yipeiling 0.05 g/L,which was more suitable for Millettia specisoa tissue culture.