益肾调气法对血管性痴呆模型大鼠脑单胺神经递质的干预作用

Intervention Effects of Tonifying Kidney and Regulating Qi Method on Cerebral Monoamine Neurotransmitters in Model Rats with Vascular Dementia

目的:通过探讨血管性痴呆(VD)大鼠脑内单胺神经递质变化,研究益肾调气法对VD大鼠的干预机制。方法:选购雄性Wistar大鼠,通过旷场试验分为正常组、卒中组。MCAO法制备缺血性脑卒中模型,常规饲养28 d后经水迷宫试验筛选VD大鼠,并随机分为观察组和模型组。正常组和模型组灌胃蒸馏水,观察组灌胃颐脑解郁方,干预4周。灌胃第1、2、4周对各组大鼠行水迷宫检测;第4周灌胃后取材,采用高效液相检测大鼠右侧海马、前额叶皮质5-HT、NE、DA含量。结果:与正常组比较,模型组大鼠1、2、4周跳台潜伏期缩短、错误次数增加、水迷宫潜伏期明显延长,差异有统计学意义(P<0.01);与模型组比较,观察组第2、4周水迷宫潜伏期缩短,差异有统计学意义(P<0.01),第4周跳台潜伏期延长、错误次数减少,差异有统计学意义(P<0.01)。模型组皮质及海马NE、DA、5-HT含量较正常组降低,差异有统计学意义(P<0.01),观察组海马DA含量较模型组升高,差异有统计学意义(P<0.01)。结论:益肾调气法可改善VD大鼠学习记忆能力,其作用机制可能与调节大鼠脑内NE、DA及5-HT含量相关。

Objective:To study the intervention mechanism of tonifying kidney and regulating qi method on vascular dementia(VD)rats by investigating the changes of monoamine neurotransmitters in the brain of VD rats.Methods:Male Wistar rats were selected and divided into a normal group and a stroke group by open field test.MCAO method was used to prepare ischemic stroke model.After 28 d of routine feeding,VD rats were screened by water maze test and randomly divided into a treatment group and a model group.The normal group and the model group were infused with distilled water,and the treatment group was infused with Yinao Jieyu Formula for 4 weeks.At the 1st,2nd and 4th weeks of gavage,the rats in each group were tested for water maze,and at the 4th week after gavage,the material was taken and the contents of 5-HT,NE,DA in the right hippocampus and prefrontal cortex of rats was detected by high-performance liquid.Results:Compared with the normal group,the latent period of 1,2 and 4 weeks of platform jumping were shortened,the number of errors increased,and the latent period of water maze was significantly prolonged(P<0.01).Compared with the model group,the latent period of water maze at 2 and 4 weeks of treatment group was shortened(P<0.01),and the latent period of platform jumping at 4 weeks was prolonged and the number of errors decreased(P<0.01).The content of NE,DA,5-HT in the cortex and hippocampus of the model group was lower than that in the normal group(P<0.01),and the hippocampal DA content in the treatment group was higher than that in the model group(P<0.01).Conclusion:The tonifying kidney and regulating qi treatment can improve the learning and memory ability of VD rats,and its mechanism may be related to the content of NE,DA and 5-HT in the rat brain.