聚吡咯氨气传感器快速检测变形杆菌

Polypyrrole Ammonia Sensor for the Detection of Bacterium Vulgare

首次提出了一种快速检测变形杆菌的聚吡咯氨气传感器。传感器采用聚吡咯作为导电电极,以代替金属电极,克服了检测系统的背景电导率限制。即使在二氧化碳气体存在下,传感器也能响应氨气浓度的变化,可用于定量检测代谢过程中释放氨气的变形杆菌。采用聚吡咯氨气传感器检测普通变形杆菌,普通变形杆菌初始浓度的对数在0.9~8.5的范围内与频率检测时间呈线性关系。与串联压电石英晶体(SPQC)传感器相比,系统具有更高的灵敏度,降低了对培养基的要求。

A new polypyrrole system was proposed to detect bacterium vulgare.In new system,polypyrrole was used as conduct electrode instead of metal electrode and overcame the background conductivity limit of detection system.Polypyrrole ammonia sensor can response the concentration changes of ammonia even in the presence of carbon dioxide.Based on this response,it can be used to detect bacterium vulgare which would release ammonia during its metabolism process.Proteus vulgaris was detected using polypyrrole ammonia sensor.The Frequency detection time and the logarithm of the initial concentration of Proteus vulgaris had a linear relationship in the range of 0.9~8.5.Compared with series piezoelectric quartz crystal(SPQC)sensor,polypyrrole ammonia sensor was more sensitive and able to reduce the requirement of culture medium.