摘要
为了最大限度获取星宿菜中的总黄酮,以星宿菜为原料,通过单因素实验和正交试验优化出提取星宿菜总黄酮含量的最佳工艺条件:35倍星宿菜的45%乙醇浓度溶解,45℃时超声提取55 min,此条件下总黄酮含量平均值为120.31 mg/g。同时测得星宿菜中黄酮的总抗氧化活性为2.266 mmol/L(FeSO4当量值)。当星宿菜中总黄酮浓度为2.75 mg/mL时,DPPH自由基清除率为85.0%。方法操作简单、快捷、灵敏度高、成本低,仪器价格及日常维护费用不高。
In order to obtain maximum limit content of total flavonoids from the Lysimachia fortune Maxim,single factor and orthogonal design test were optimized.The optimizing conditions were obtained,which were 45%of ethanol concentration,1∶35(g/mL)of feed liquid ratio,55 min of ultrasonic time and 45°C of ultrasonic temperature.The average content value of total flavonoids was 120.31 mg/g.Meanwhile,the total antioxidant activity of total flavonoids in the Lysimachia fortune Maxim was 2.266 mmol/L(FeSO4 equivalent value).The free radical scavenging rate of DPPH was 85.0%when the concentration of total flavonoids in the Lysimachia fortune Maxim was 2.75 mg/mL.The method was simple,rapid,high sensitivity and low-cost.In addition,the instrument price and maintenance cost were not high.
作者
郭蒙
韦启道
刘韬
田琴
杨再波
GUO Meng;WEI Qi-dao;LIU Tao;TIAN Qin;YANG Zai-bo(School of Chemistry and Chemical Engineering,Qiannan Normal College for Nationalities,Duyun 558000,China;Engineering Research Center for Development of Ethnic Medicinal Plant Resources in Colleges and Universities of Guizhou Province,Duyun 558000,China)
出处
《化学试剂》
CAS
北大核心
2020年第5期584-588,共5页
Chemical Reagents
基金
贵州省教育厅青年科技人才成长项目(黔教合KY字[2018]440)
黔南师院高层次人才研究专项项目(qnsyrc201503)
贵州省普通高校民族药用植物资源开发工程研究中心开放基金资助项目(黔教合KY字[2014]227)。
关键词
总黄酮
正交设计
星宿菜
超声提取
抗氧化活性
total flavonoids
orthogonal design
Lysimachia fortune Maxim
ultrasonic extraction
antioxidant activity
