血清IL-1、IL-2、IL-6、IL-8、IL-10和hs-CRP水平与新生血管性青光眼的相关性

Correlations of serum IL-1,IL-2,IL-6,IL-8,IL-10 and hs-CRP levels with neovascularization glaucoma

目的探讨血清白细胞介素(IL)-1、IL-2、IL-6、IL-8、IL-10及高敏C反应蛋白(hs-CRP)水平与新生血管性青光眼(NVG)患者的相关性。方法选取NVG患者69例(NVG组)和体检健康者39名(正常对照组),检测所有对象的血清IL-1、IL-2、IL-6、IL-8、IL-10及hs-CRP水平,同时收集体格检查资料及眼科检查资料[眼压(IOP)、中央角膜厚度(CCT)、垂直杯盘比(VCDR)、眼轴长度(AL)、前房深度(ACD)、平均视野缺损(MD)、平均视敏度(MS)等]。将NVG患者根据病因分为视网膜静脉阻塞(RVO)继发性NVG组(32例)和糖尿病视网膜病变(DR)继发性NVG组(37例)。采用Spearman相关分析评估各项指标与NVG患者眼部参数的相关性。采用二分类Logistic回归分析评估NVG发生的危险因素。采用受试者工作特征(ROC)曲线分析各项指标诊断NVG的价值。结果NVG组血清IL-6、IL-8和hs-CRP水平显著高于正常对照组(P<0.01),血清IL-1、IL-2和IL-10水平2个组之间差异均无统计学意义(P>0.05)。DR继发性NVG组与RVO继发性NVG组之间IL-1、IL-2、IL-6、IL-8、IL-10和hs-CRP水平差异均无统计学意义(P>0.05)。Spearman相关分析结果显示,NVG患者血清IL-6水平与MD呈正相关(r=0.257,P<0.001),与MS呈负相关(r=-0.196,P<0.001),血清IL-1、IL-2、IL-8、IL-10、hs-CRP水平与眼部参数均无相关性(P>0.05)。二分类Logistic回归分析结果显示,IL-6[比值比(OR)=4.443,95%可信区间(CI)为1.207~16.40]、IL-8(OR=1.067,95%CI为1.001~1.237)、hs-CRP(OR=3.886,95%CI为1.191~12.683)是NVG发病的危险因素。ROC曲线分析结果显示,IL-6、IL-8、hs-CRP和三者联合检测诊断NVG的曲线下面积分别为0.773、0.873、0.760、0.902。联合检测的诊断效能优于单项检测。结论IL-6、IL-8和hs-CRP对NVG均有一定的辅助诊断价值,可能与NVG的发生、发展有一定关系。

Objective To investigate the correlations of serum interleukin(IL)-1,IL-2,IL-6,IL-8,IL-10 and high-sensitivity C-reactive protein(hs-CRP)levels with neovascularization glaucoma(NVG).Methods A total of 69 patients with NVG and 39 healthy subjects were enrolled.The levels of IL-1,IL-2,IL-6,IL-8,IL-10 and hs-CRP were determined.Ophthalmic examination[intraocular pressure(IOP),central corneal thickness(CCT),vertical cup-disc ratio(VCDR),axial length(AL),anterior chamber depth(ACD),visual field mean defect(MD),visual field mean sensitivity(MS)and so on]and physical examination were performed.There were 32 cases of retinal vein occlusion(RVO)secondary NVG and 37 cases of diabetic retinopathy(DR)secondary NVG.Spearman correlation analysis was used to evaluate the correlation between factors and ophthalmic parameters of NVG patients.Binary Logistic regression analysis was performed to identify the risk factors for NVG.Receiver operating characteristic(ROC)curve was used to analyze the value of each parameter in diagnosing NVG.Results Serum levels of IL-6,IL-8 and hs-CRP in NVG group were higher than those in control group(P<0.01).There was no statistical significance in IL-1,IL-2 and IL-10 between the 2 groups(P>0.05).There was no statistical significance in IL-1,IL-2,IL-6,IL-8,IL-10 and hs-CRP levels between DR secondary NVG group and RVO secondary NVG group(P>0.05).Spearman correlation analysis showed that serum IL-6 level was positively correlated with MD in NVG patients(r=0.257,P<0.001),and it was negatively correlated with MS(r=-0.196,P<0.001).Serum IL-1,IL-2,IL-8,IL-10 and hs-CRP levels were not correlated with ophthalmic parameters(P>0.05).Binary Logistic regression analysis showed that IL-6[odds ratio(OR)=4.443,95%confidence interval(CI)1.207-16.40],IL-8(OR=1.067,95%CI 1.001-1.237)and hs-CRP(OR=3.886,95%CI 1.191-12.683)were independent risk factors of NVG.ROC curve showed that the areas under curves of IL-6,IL-8,hs-CRP and the combined determination(IL-6+IL-8+hs-CRP)were 0.773,0.873,0.760 and 0.902,respectively.The efficiency of the combined determination was better than those of single determinations.Conclusions IL-6,IL-8 and hs-CRP are potential for the diagnosis of NVG,which play roles in the development of NVG.