摘要
目的探讨使用UF-1000i尿沉渣分析仪检测白细胞及小圆上皮细胞假性升高的主要原因。方法随机收集门诊尿液标本,分别用UF-1000i尿沉渣分析仪和显微镜进行检测,对2种方法检测结果进行统计学分析,分析导致UF-1000i尿沉渣分析仪检测白细胞及小圆细胞假性升高的影响因素。结果UF-1000i尿沉渣分析仪检测白细胞的假阳性率为12.58%,导致假阳性的因素主要包括小圆上皮细胞(69.6%)、细菌(12.8%)、扁平上皮细胞(8.0%)和脂肪滴(6.4%);检测小圆上皮细胞的假阳性率为4.6%,导致假阳性的因素主要包括白细胞(67.9%)、脂肪滴(15.5%)和滴虫(11.9%)。结论当尿液病理成分增加时,多种因素可以影响UF-1000i尿沉渣分析仪对白细胞和小圆上皮细胞的检测结果,因此应用UF-1000i尿沉渣分析仪检测只能作为初步筛查方法,显微镜镜检仍然是尿沉渣检查的金标准。
Objective To investigate the main causes for false increasing of white blood cells and small round epithelial cells determined by UF-1000i urine sediment analyzer.Methods Urine samples of outpatients were collected.UF-1000i urine sediment analyzer and microscopy were used,and the results of these 2 methods were compared.The main causes for false increasing of white blood cells and small round epithelial cells were evaluated.Results The false positive rate of white blood cells determined by UF-1000i urine sediment analyzer was 12.58%,mainly due to small round epithelial cells(69.6%),bacteria(12.8%),flat epithelial cells(8.0%)and fat droplets(6.4%).The false positive rate of small round epithelial cells was 4.6%,mainly due to leukocytes(67.9%),fat droplets(15.5%)and trichomoniasis(11.9%).Conclusions When the urine pathological components increase,a variety of factors can affect UF-1000i urine sediment analyzer's determination results on white blood cells and small round epithelial cells,and false positivity appears.The application of UF-1000i urine sediment analyzer can only be a determination as a preliminary screening method,and microscopy is still be gold standard for urine sediment analysis.
作者
周忻
张珏
陈莉英
ZHOU Xin;ZHANG Jue;CHEN Liying(Shuguang Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)
出处
《检验医学》
CAS
2020年第5期444-446,共3页
Laboratory Medicine