西藏青稞抗白粉病的广泛靶向代谢组研究

A Wide Range of Targeted Metabolome Studies on Resistance toPowdery Mildew in Qingke Barley

代谢物是生物体表型的基础,尤其次生代谢物的研究能帮助更直观有效地了解生物学过程及其机理。基于对代谢物的定性定量分析,代谢组学可以用于代谢途径或代谢网络的解析。本研究基于广泛靶向代谢组技术的代谢分析,定性定量多种西藏青稞叶片中的代谢产物,为揭示青稞抗白粉病的代谢机理提供理论支撑。本研究选取36个西藏青稞抗白病样品,分为18组进行代谢研究,每组样品为2个生物学重复。采⽤UPLC-MS/MS检测平台、迈维代谢生物科技有限公司的数据库以及多元统计分析相结合的手段研究样品间代谢组。本实验共检测到751个代谢产物,已确定出30种类型的代谢物,数量最多的五种代谢物类型依次分别为黄酮(163个)、黄酮碳糖苷(77个)、有机酸及其衍生物(65个)、氨基酸衍生物(55个)、核苷酸及衍生物(55个)。抗白粉青稞叶片中黄酮含量远高于其它代谢物,黄酮碳糖苷含量次之。这些发现有助于提高我们对青稞抗白粉病在代谢层面的认知,将为西藏青稞抗白粉病品种的开发提供新思路。

Metabolites are the basis of an organism's phenotype,and the study of metabolites can help to understand biological processes and mechanisms more intuitively and effectively.Based on qualitative and quantitative analysis of metabolites,metabolomics can be used to analyze metabolic pathways or metabolic networks.This study was based on the metabolic analysis of a wide range of targeted metabolomics techniques.The qualitative and quantitative analysis of metabolites in various Tibetan highland barley leaves provided theoretical support for revealing the molecular mechanism of highland barley resistance to powdery mildew.In this study,36 samples of Tibetan highland barley resistant to powdery mildew were selected and divided into 18 groups for metabolic study,with two biological duplicates in each group.UPLC/MS/MS detection platform,Metaware metabolism of biological science and technology co.,Ltd.database and multivariate statistical analysis method were used to study group sample metabolism.In this study,a total of 751 metabolites were detected,and 30 types of metabolites were identified.The five metabolites with higher content were flavonoids (163), flavonoids carbonyl glycosides (77), organic acids andtheir derivatives (65), amino acid derivatives (55), nucleotides and derivatives (55), respectively.The content of flavonoids in the leaves of highland barley resistant to powdery mildew in Tibetwas much higher than that of other metabolites, followed by the content of flavonoids glycosides.These findings help to improve our understanding of the metabolic level of resistance to powderymildew in Qingke barley and will provide new ideas for the development of resistance to powderymildew in Tibetan hulless barley.