TGIF1表达水平对乳腺癌细胞上皮型钙黏附蛋白和Twist1蛋白表达的影响

Effect of TGIF1 expression on epithelial cadherin and Twist1 protein expression in breast cancer cells

目的:观察转化生长因子β-同源诱导因子1(TGIF1)表达水平对乳腺癌细胞上皮型钙黏附蛋白(E-cadherin)和人Twist相关蛋白1(Twist1)表达的影响。方法:24只6周龄SPF级Balb/c雌性小鼠随机分为对照组、TGIF1沉默组、TGIF1正常组和TGIF1过表达组,TGIF沉默组小鼠给予慢病毒颗粒TGIF shRNA (h) lentiviral particles(sc-36,659-V)干扰的4T1乳腺癌细胞构建乳腺癌小鼠模型,TGIF1正常组小鼠采用乳腺癌细胞(4T1)构建乳腺癌小鼠模型,TGIF1过表达组采用TGIF1过表达的4T1乳腺癌细胞建立乳腺癌小鼠模型,测定各组小鼠乳腺肿瘤组织中TGIF1、E-cadherin和Twist1蛋白水平。结果:TGIF1过表达组小鼠肿瘤体积明显大于TGIF1正常组和沉默组,组间差异比较有统计学意义(P<0.05);TGIF1正常组、TGIF1沉默组、TGIF1过表达组TGIF1和Twist1蛋白表达水平明显高于对照组,E-cadherin明显低于对照组,组间差异比较有统计学意义(P<0.05);TGIF1沉默组TGIF1和Twist1蛋白表达水平明显低于TGIF1正常组,E-cadherin明显高于TGIF1正常组,组间差异比较有统计学意义(P<0.05);TGIF1过表达组TGIF1和Twist1蛋白表达水平明显高于TGIF1正常组,E-cadherin明显低于TGIF1正常组,组间差异比较有统计学意义(P<0.05);Pearson相关性分析结果显示,乳腺癌组织TGIF1表达水平与E-cadherin蛋白表达水平呈明显负相关,与Twist1蛋白水平呈明显正相关(P<0.05)。结论:TGIF1可通过调控E-cadherin和Twist1干预EMT途径从而影响乳腺癌的转移和侵袭,值得进一步研究。

Objective:To observe the effect of the expression of transforming growth factorβ-homologous inducible factor 1(TGIF1)on the expression of epithelial cadherin(E-cadherin)and human Twist-related protein 1(Twist1)in breast cancer cells.Methods:Totally 246-week-old female SPF Balb/c mice were randomly divided into a control group,a TGIF1 silencing group,a TGIF1 normal group,and a TGIF1 overexpression group.In the TGIF1 silenced group,4T1 breast cancer cells were interfered by lentivirus shRNA(H)lentiviral particles(sc-36659-v)to construct a breast cancer model.TGIF1 normal mice used breast cancer cells(4T1)to construct a mouse model of breast cancer.Mice in the TGIF1 silencing group used lentiviral particles TGIF1 shRNA(h)lentiviral particles(sc-36,659-V)to interfere with 4T1 breast cancer cells and the normal TGIF group used normal 4T1 breast cancer cells and the TGIF overexpression group used 4T1 breast cancer cells with TGIF1 overexpression to establish a mouse model of breast cancer.Determination of TGIF1,E-cadherin and Twist1 protein levels in breast tumor tissue of mice in each group.Results:The tumor volume of mice in the TGIF1 overexpression group was significantly larger than that in the TGIF1 normal group and the silent group,and the differences between the groups were statistically significant(P<0.05).The expression levels of TGIF1 and Twist1 protein in TGIF1 normal group,TGIF1 silent group,and TGIF1 overexpression group were significantly higher than those in control group,and E-cadherin was significantly lower than that in control group.The differences between groups were statistically significant(P<0.05).The expression level of TGIF1 and Twist1 protein in TGIF1 silenced group was significantly lower than that in TGIF1 normal group,and E-cadherin was significantly higher than that in TGIF1 normal group(P<0.05).The expression levels of TGIF1 and Twist1 proteins in the TGIF1 overexpression group were significantly higher than those in the TGIF1 normal group,and E-cadherin was significantly lower than that in the TGIF1 normal group.The differences between the groups were statistically significant(P<0.05).Pearson correlation analysis showed that the expression level of TGIF1 in breast cancer tissue was significantly negatively correlated with the expression level of E-cadherin protein,and was significantly positively correlated with the level of Twist1 protein(P<0.05).Conclusion:TGIF1 can affect the metastasis and invasion of breast cancer by regulating E-cadherin and Twist1 to interfere with the EMT pathway,which deserves further study.