三种方法检测肺炎支原体抗体的一致性分析

Consistency analysis of chemiluminescent immunoassay and gelatin particle agglutination test, enzyme-linked immunosorbent assay for detection of mycoplasma pneumoniae antibody

目的探究吖啶酯化学发光法(CLIA)、酶联免疫吸附法(ELISA)和明胶颗粒凝集法(GPA)三种检测肺炎支原体(MP)抗体的一致性,以确定临床使用以上方法进行筛查的可行性。方法 1404例疑似小儿肺炎支原体肺炎(MPP)患儿,分别使用CLIA、ELISA和GPA三种方法检测其血清中MP抗体,分析CLIA、ELISA和GPA检测结果的一致性。结果 CLIA与ELISA检测MP-Ig G、MP-Ig M抗体结果比较差异具有统计学意义(χ^2=9.095、4.272,P<0.05)。CLIA与ELISA检测MP抗体结果比较差异具有统计学意义(χ^2=18.712,P<0.05)。CLIA与GPA检测MP抗体的Kappa值=0.626,总符合率为82.05%,具有一致性。ELISA与GPA检测MP抗体结果比较差异具有统计学意义(χ^2=48.463,P<0.05)。ELISA与GPA检测MP抗体的Kappa值=0.496,总符合率为75.07%,具有一致性。结论采用CLIA、GPA和ELISA检测MP抗体诊断儿童MPP均有一定的价值,三种方法具有一致性,可用于早期MPP的检测。

Objective To investigate the consistency of chemiluminescent immunoassay(CLIA)and gelatin particle agglutination(GPA)test,enzyme-linked immunosorbent assay(ELISA)for detection of mycoplasma pneumoniae(MP)antibody,so as to determine the feasibility of the above methods for screening.Methods 1404 children suspected of mycoplasma pneumoniae pneumonia(MPP)were tested for MP antibody by three methods of CLIA,ELISA and GPA,and the consistency of CLIA,ELISA and GPA test results was analyzed.Results The difference between CLIA and ELISA in detecting MP-Ig G and MP-Ig M antibodies is statistically significant(χ^2=9.095,4.272,P<0.05).The difference between CLIA and ELISA in detecting MP antibody is statistically significant(χ^2=18.712,P<0.05).Kappa value of MP antibody detected by CLIA and GPA was 0.626,the total coincidence rate was 82.05%and had consistency.The difference between ELISA and GPA in detecting MP antibody is statistically significant(χ^2=48.463,P<0.05).Kappa value of MP antibody detected by ELISA and GPA was 0.496,the total coincidence rate was 75.07%and had consistency.Conclusion Application of CLIA,GPA and ELISA for detection of MP antibodies to diagnose MPP in children has certain value.The three methods are consistent and can be used for early MPP detection.