女性宫颈HPV感染与阴道局部Th17细胞免疫功能及血清因子的关系研究

Study on the relationship between cervical HPV infection and local Th17 cell immune function in women

目的探讨女性宫颈人乳头瘤病毒(HPV)感染与阴道局部辅助性T细胞17(Th17)细胞免疫功能的关系。方法选取2017年1月至2018年12月在本院就诊的HPV感染阳性者182例,其中高危型HPV感染者78例,低危型HPV感染者104例;高危型HPV感染者中,持续感染者26例,非持续感染者52例;检测Th17细胞及白细胞介素⁃17(IL⁃17)、IL⁃10、IL⁃6、IL⁃4和转化生长因子⁃b(TGF⁃b)。结果HPV阳性者Th17细胞、IL⁃17、IL⁃6、IL⁃4和TGF⁃b分别为(10.03±3.10)%、(159.82±31.33)ng/L、(46.60±10.61)ng/L、(150.03±31.15)ng/L和(4.40±13.32)ng/L,明显高于HPV阴性者,差异有统计学意义(P<0.05);高危型HPV感染者IL⁃10和IL⁃4分别为(21.12±3.72)ng/L和(142.21±30.02)ng/L,明显低于低危型HPV感染者(P<0.05);高危型HPV感染和低危型HPV感染者Th17细胞、IL⁃17、IL⁃6和TGF⁃b水平比较差异无统计学意义(P<0.05);高危型HPV持续感染和非持续感染者Th17细胞、IL⁃17、IL⁃10、IL⁃6、IL⁃4和TGF⁃b水平比较差异无统计学意义(P<0.05)。结论女性宫颈HPV感染可明显影响阴道局部Th17细胞免疫功能,值得进一步研究。

Objective To investigate the relationship between cervical human papillomavirus(HPV)infection in women and the immune function of local helper T cell 17(Th17)in vagina.Methods From January 2017 to December 2018,182 cases of HPV infection were selected,including 78 cases of high⁃risk HPV infection and 104 cases of low⁃risk HPV infection.Among the high⁃risk HPV infections,26 were persistent and 52 were non⁃persistent.Th17 cells and interleukin⁃17(IL⁃17),IL⁃10,IL⁃6,IL⁃4 and transforming growth factor⁃β(TGF⁃β)were measured.Results Th17 cells,IL⁃17,IL⁃6,IL⁃4 and TGF⁃βin HPV positive patients were(10.03±3.10)%,(159.82±31.33)ng/L,(46.60±10.61)ng/L,(150.03±31.15)ng/L and(4.40±13.32)ng/L,significantly higher than those in HPV negative patients(P<0.05);The levels of IL⁃10 and IL⁃4 in high⁃risk HPV patients were(21.12±3.72)ng/L and(142.21±30.02)ng/L,significantly lower than those in low⁃risk HPV patients(P<0.05).There was no significant difference in the levels of Th17 cells,IL⁃17,IL⁃6 and TGF⁃βbetween high⁃risk HPV infection and low⁃risk HPV infection(P<0.05).There were no significant difference in the levels of Th17 cells,IL⁃17,IL⁃10,IL⁃6,IL⁃4 and TGF⁃βbetween persistent and non⁃persistent high⁃risk HPV infections(P>0.05).Conclusion HPV infection in the cervix of women can significantly affect the immune function of Th17 cells in local vagina.It is worth further investigation.