摘要
植物油中甘油三酯种类众多、结构复杂,准确定性定量非常困难。本文采用液相色谱-串联质谱法,选择反应监测扫描模式下,两对离子对定性、外标法准确定量植物油中七种主要甘油三酯,包括:三亚油酸甘油酯(LLL)、1-油酸-2,3-亚油酸甘油酯(OLL)、1-棕榈酸-2,3-亚油酸甘油酯(PLL)、1-棕榈酸-2-油酸-3-亚油酸甘油酯(POL)、三油酸甘油酯(OOO)、1,2-油酸-3-棕榈酸甘油酯(OOP)、1-棕榈酸-2-油酸-3-硬脂酸甘油酯(POS)。使用反相色谱柱分离甘油三酯,以异丙醇/乙腈混合溶液和乙腈/水混合溶液为流动相梯度洗脱,15 min内可实现甘油三酯的分离与检测。该方法线性关系良好,均在0.996以上,检出限为0.3μg/kg,定量限为1.0μg/kg,精密度(RSD)为1.2%~4.9%。该方法操作简单、快速,能够实现菜籽油、玉米油、花生油中七种甘油三酯的准确定量。
There are many types of triglycerides in vegetable oils,and their structures are complex,which is difficult to quantify with certainty.This paper used liquid chromatography-tandem mass spectrometry,selective re⁃action monitoring(SRM)mode,two pairs of ion pairs for qualitative,external standard method to accurately quantify seven major triglycerides in vegetable oils,including:glyceryl trilinolein(LLL),1-oleic acid-2,3-linoleic acid glyc⁃eride(OLL),1-palmitic acid-2,3-linoleic acid glyceride(PLL),1-palmitic acid-2-oleic acid-3-linoleic acid glyc⁃eride(POL),glyceryl trioleate(OOO),1,2-oleate-3-glycerate glyceride(OOP),1-palmitic acid-2-oleic acid-3-stearic acid glyceride(POS).The triglycerides were separated by reversed-phase chromatography,and the isopropyl alcohol/acetonitrile mixed solution and acetonitrile/water mixed solution were used as mobile phase gradient elu⁃tion.The triglyceride could be separated and detected within 15 minutes.This method has a good linear relation⁃ship,all above 0.996,with a detection limit of 0.3μg/kg,a limit of quantification of 1.0μg/kg,and a precision(RSD)of 1.2%to 4.9%.The method is simple and fast to operate,and it can be used to detect the seven triglycerides in vegetable oils such as rapeseed oil,corn oil,and peanut oil.
作者
赵新楠
王秀嫔
李培武
印南日
万立昊
王晓
张良晓
ZHAO Xin-nan;WANG Xiu-pin;LI Pei-wu;YIN Nan-ri;WAN-Li hao;WANG Xiao;ZHANG Liang-xiao(Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China;Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Wuhan 430062,China;Laboratory of Quality and Safety Risk Assessment for Oilseed Products(Wuhan),Ministry of Agriculture and Rural Affairs,Wuhan 430062,China;Quality Inspection and Test Center for Oilseed Products,Ministry of Agriculture and Rural Affairs,Wuhan 430062,China;Henan University of Technology,Zhengzhou 450001,China)
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2020年第3期356-363,共8页
Chinese Journal of Oil Crop Sciences
基金
中国农科院基本科研项目(粮油风险因子全景筛查技术研究)(1610172019007)。
关键词
植物油
甘油三酯
液相色谱-串联质谱
准确定量
vegetable oils
triglycerides
liquid chromatography-tandem mass spectrometry
accurate quanti⁃fication