预培养条件对向日葵列当种子萌发的影响

Effects of preculture conditions on the germinationof sunflower broomrape seeds

为进一步探讨向日葵列当萌发机理,完善向日葵列当防除方法,设置不同的温度、渗透势、预培养时间、pH值、赤霉素浓度等预培养条件处理,进行单因素试验及正交试验,研究预培养条件对向日葵列当萌发的影响。结果表明,各因素单独作用下,预培养阶段最适的环境条件为:温度25℃,渗透势0MPa,预培养时间7 d,p H 7.0,赤霉素浓度30 mg/m L;交互作用下,影响向日葵列当种子萌发的4个因素主次顺序为预培养温度>预培养时间>渗透势>p H,其中预培养温度和预培养时间对向日葵列当种子萌发具有显著影响,最适宜的预培养条件组合为:预培养温度25℃,渗透势为-0.5 MPa,p H为7.0的条件下预培养7 d,该组合下萌发率最高,为75.85%。

In order to study the effects of preculture conditions on germination of sunflower broomrape seeds,different preculture conditions,including temperature,osmotic potential,preculture time,pH and gibberellin con⁃centration,were set to conduct one element experiments and orthogonal design experiments.The one element experi⁃ment results showed that the optimal preculture conditions were temperature at 25℃,preculture time at 7 days,os⁃motic potential at 0 MPa,pH 7.0,with concentration of gibberellins of 30 mg/mL.The orthogonal design experi⁃ment results showed that,the primary and secondary influence order of the elements which affect the germination of sunflower broomrape seeds was preculture temperature>preculture time>osmotic potential>pH.The optimal pre⁃culture conditional combination included temperature at 25℃,preculture time at 7 days,osmotic potential at-0.5 MPa,pH 7.0.The germination rate of sunflower broomrape seeds reached the highest of 75.85%at the optimal preculture conditional combinations.