摘要
目的优选党参多糖最佳提取工艺。方法通过单因素试验法和L9(34)正交试验法,采用苯酚-硫酸法测定吸光度值,以多糖提取率为评价指标,对提取工艺进行优选。结果最佳提取工艺是醇沉浓度为85%,加热回流提取3次,液固比200∶1,每次提取2h。结论筛选得到的提取工艺简便易行,稳定性好。
OBJECTIVE To optimize the extraction process of codonopsis pilosula polysaccharides.METHODS By single factor test and L9(34) orthogonal test,the absorbance value was determined by phenol-sulfuric acid method,and the extraction rate of polysaccharides was used as the evaluation index to optimize the extraction process.RESULTS The optimum extraction process was 85% alcohol precipitation concentration,3 times heated reflux extraction,the ratio of liquid to solid was 200∶1,2 h each time.CONCLUSION The optimized extraction process is very simple and stable.
作者
吕立铭
申茹
李大炜
麦艳珍
高娟
LV Li-ming;SHEN Ru;LI Da-wei;MAI Yan-zhen;GAO Juan(Huizhou Health Sciences Polytechnic,Huizhou 516025,China)
出处
《海峡药学》
2020年第4期56-58,共3页
Strait Pharmaceutical Journal
基金
广东省中医药局面上科研项目(NO.20191341)
惠州市科技计划医疗卫生项目(NO.2016Y120)
惠州市科技计划项目(C2014C15)。
关键词
党参
党参多糖
提取工艺
正交实验
Codonopsis Radix
Polysaccharides
Extraction process
Orthogonal design method
