辣木叶多糖的提取及抗氧化活性研究

Study on Extraction Technology and Antioxidant Activity of Polysaccharide from Moringa oleifera

为提取辣木叶多糖并研究其体外抗氧化活性,利用水提醇沉法获得辣木叶粗多糖,以单因素提取试验为基础,采用正交试验对辣木叶多糖的分离提取工艺进行优化,并探讨6个产地辣木叶粗多糖体外抗氧化活性。研究表明,辣木叶粗多糖的最佳提取工艺:液料比20∶1(mL/g),提取温度为70℃,提取时间为1.5 h,在此工艺条件下多糖的得率为11.48%。6个产地辣木叶粗多糖以普洱市辣木叶多糖的体外抗氧化活性最佳。

In order to extract crude polysaccharides in the leaves of Moringa oleifera and study its in-vitro antioxidation activity,on the basis of the single factor extraction experiment,this research used ethanol subsiding method to get crude polysaccharides in the leaves of Moringa oleifera,optimized techniques of polysaccharide extraction with orthogonal experiment and analyzed in-vitro antioxidation activity of crude polysaccharide of the leaves of Moringa oleifera in 6 areas.The research showed that the best extracting processes were as follows,liquid to solid ratio was 20∶1(mL/g),extracting temperature was 70℃and extracting time was 1.5 h.Under such conditions,the final productivity of polysaccharides was up to 11.48%.The in-vitro antioxidation activity of crude polysaccharide of the the leaves of Moringa oleifera in Puer was the best among 6 areas.