前列腺素E2受体拮抗剂对肝癌细胞增殖迁移及EP4R/Snail和EP4R/c-myc表达的影响观察

Effects of prostaglandin E2 receptor antagonist CJ-42794 on proliferation,migration,and Snail and c-myc expression of hepatocellular carcinoma cells

目的观察前列腺素E2受体拮抗剂CJ-42794对肝癌细胞增殖迁移及EP4R/Snail和EP4R/c-myc表达的影响。方法将肝癌细胞HepG2和Huh7均分别随机分为观察组、PGE2组、对照组,观察组加入5μmol/L的PGE2+5μmol/L的cj-42794,PGE2组分别加1、2、5μmol/L的PGE2(A,B,C组),对照组加入相同体积溶媒。观察组加药处理24 h、48 h用MTT法检测细胞活力,用Transwell法检测处理48 h侵袭的细胞数,用Western blotting法检测两种细胞中Snail、c-myc。结果HepG2细胞中,培养24 h时,与对照组比较,B、C组和观察组的细胞增殖率升高,且以C组升高最显著(P均<0.05),与B组比较,观察组细胞增殖率降低(P>0.05);培养48 h时,与对照组比较,B、C组细胞增殖率升高,且以C组升高最显著(P<0.05),与B、C组比较,观察组的细胞增殖率降低(P<0.05)。Huh7细胞中,培养24 h时,与对照组比较,B、C组和观察组的细胞增殖率升高,C组升高最显著(P<0.05),与C组比较,观察组细胞增殖率低(P<0.05);培养48 h时,与对照组相比,C组的细胞增殖率升高(P<0.05),与B、C组比较,观察组的细胞增殖率降低(P<0.05)。HepG2、Huh7细胞中,与对照组比较,PGE2各刺激组的迁移细胞数均增多(P<0.05),与B、C组比较,观察组的细胞迁移细胞数减少(P均<0.05)。两种细胞中,与对照组比较,PGE2组c-myc、Snail蛋白表达水平均升高(P均<0.05)。与PGE2组比较,观察组c-myc、Snail蛋白表达水平下调(P均<0.05)。结论前列腺素E2受体拮抗剂CJ-42794可抑制肝癌细胞增殖迁移,且抑制EP4R/Snail、EP4R/c-myc信号通路的表达。

Objective To explore the effects of prostaglandin E2 receptor antagonist CJ-42794 on the proliferation,migration,and Snail and c-myc expression of hepatocellular carcinoma(HCC)cells.Methods HCC cells HepG2 and Huh7 were randomly divided into the observation group,PGE2 group,and control group,respectively.Fiveμmol/L PGE2+5μmol/L cj-42794 was added to the observation group,1,2,and 5μmol/L PGE2 was added to the PGE2 group(A,B and C groups),and meanwhile,the same volume of solvent was added to the control group.In the observation group,after 24 and 48 hours of treatment,MTT was used to detect the cell vitality,Transwell method to detect the number of invasive cells,and Western blotting to detect the expression of Snail and c-myc.Results In the HepG2 cells,at 24 h after culture,the cell proliferation rates of group B,group C,and observation group increased compared with that of the control group,with the most significant increase in the group C(P<0.05).Compared with group B,the cell proliferation rate of observation group decreased(P<0.05);at 48 h,compared with the control group,the cell proliferation rates of group B and C increased,with the most significant increase in the group C(P<0.05);compared with groups B and C,the cell proliferation rate of the observation group decreased(P<0.05).In the Huh7 cells,at 24 h,compared with the control group,the cell proliferation rates of group B,group C,and observation group increased,with the most significant increase in the group C(all P<0.05).Compared with the group C,the cell proliferation rate of observation group was low(P<0.05).At 48 h,the cell proliferation rate of group C increased as compared with that of the control group(P<0.05),while the cell proliferation rate of the observation group decreased as compared with those of groups B and C(both P<0.05).In hepG2 and Huh7 cells,compared with the control group,the number of migration cells in each PGE2 stimulation group increased(P<0.05),and the number of migration cells in the observation group was significantly inhibited compared with those of the groups B and C(P<0.05).Conclusion Prostaglandin E2 receptor antagonist CJ-42794 can inhibit the proliferation and migration of liver cancer cells and inhibit the expression of Snail and c-myc.