吲哚-3-甲醇灌胃对小鼠变应性鼻炎治疗作用观察

Effect of intragastric administration of indole-3-carbinol on allergic rhinitis mice

目的观察肠道代谢产物吲哚-3-甲醇(I3C)灌胃对小鼠变应性鼻炎的治疗作用。方法将BALB/c小鼠随机分成干预1组、干预2组、干预3组、干预对照组、模型组、正常组。除正常组外其余组均接受卵清蛋白及氢氧化铝佐剂腹腔注射,隔天1次,注射7次,第15天再以5%卵清蛋白溶液滴鼻,1次/d,连续7 d,建立变应性鼻炎模型,正常组接受等量生理盐水腹腔注射和滴鼻。干预1、2、3组建模成功后分别以I3C 0.8、0.4、0.2 mg/d混合在橄榄油与无水乙醇里灌胃1周,干预对照组建模成功后用等量的橄榄油与无水乙醇混合液灌胃。各组造模成功后及灌胃1周后观察小鼠行为学表现,鼻前部组织病理切片观察炎性细胞量;流式细胞仪检测外周血Tregs/Th17;ELISA法检测血清IL-10、IgE;结果与模型组比较,干预1、2、3组症状评分降低(P均<0.05);干预对照组、模型组比较,P>0.05;干预1、干预2、干预3组间两两比较,P均>0.05。正常组、干预1组、干预2组、干预3组鼻前部组织结构完整,上皮细胞排列整齐,无明显的炎性细胞浸润。模型组和干预对照组鼻前部组织结构紊乱,间质见充血及增生,见大量炎性细胞增生。与正常组比较,模型组、干预对照组血清Tregs所占百分比、IL-10水平降低,Th17细胞所占百分比、IgE水平升高(P均<0.05);与模型组比较,干预1、2、3组血清Tregs百分比、IL-10水平升高,Th17细胞所占百分比、IgE水平降低(P均<0.05);干预对照组与模型组各指标比较,P均>0.05;干预1、2、3组之间各指标两两比较,P均>0.05。结论I3C可改善变应性鼻炎小鼠临床症状及组织病理表现,其作用机制可能是平衡Tregs/Th17细胞百分比、增加血清IL-10水平、降低IgE水平。

Objective To observe the therapeutic effect of indole-3-carbinol(I3C)on allergic rhinitis mice.Methods BALB/c mice were randomly divided into six groups:intervention group 1,intervention group 2,intervention group 3,intervention control group,model group,and normal group.Mice in all groups except the normal group were injected intraperitoneally with ovalbumin and aluminum hydroxide adjuvant,once every other day,for 7 times,and on the 15th day,the noses were dripped by 5%ovalbumin solution once a day,for 7 consecutive days.The mice in the normal group were given the same amount of normal saline through intraperitoneal injection and nasal drip.In the intervention groups 1,2,and 3,after mice were successfully modeled,they were given olive oil and anhydrous ethanol for 1 week with I3C mixture of 0.8,0.4 and 0.2 mg/day,respectively.After the intervention control models were successfully established,they were given the mixture of olive oil and anhydrous ethanol.Mice′s behaviors were observed after successful modeling and at 1 week after gavage;the inflammatory cells were observed by pathological section of anterior nasal tissues;Tregs/Th17 of peripheral blood was detected by flow cytometry;IL-10 and IgE were detected by ELISA.Results Compared with the model group,intervention groups 1,2,and 3 had lower symptom scores(all P<0.05);no significant difference was found between the intervention control group and the model group,and between the intervention group 1,intervention group 2,and intervention group 3(all P>0.05).The anterior nasal tissue structure of the normal group,intervention group 1,intervention group 2,and intervention group 3 was complete,and epithelial cells were arranged neatly,with no obvious inflammatory cell infiltration.The anterior nasal tissues structure of the model group and the control group was disordered,with hyperemia and hyperplasia of the interstitial tissues,and a large number of inflammatory cells.Compared with the normal group,the percentage of serum Tregs and the IL-10 level of the normal group and intervention control group decreased,and the percentage of Th17 cells and the level of IgE increased(all P<0.05).Compared with model group,the percentage of serum Tregs and the IL-10 level of the intervention groups 1,2,and 3 increased,and the percentage of Th17 cells and the level of IgE decreased(all P<0.05).There was no significant difference between the control group and the model group,and between the intervention groups 1,2,and 3(all P>0.05).Conclusion I3C may improve the clinical symptoms and histopathological features of allergic rhinitis mice by balancing the percentage of Tregs/Th17 cells,increasing serum IL-10 level and lowering IgE level.