TNF-α诱导的circMAN1A2促进宫颈癌细胞的迁移、侵袭和增殖

TNF-α-induced circMAN1A2 promotes migration,invasion and proliferation of cervical cancer cells

目的:探讨肿瘤坏死因子(TNF)-α诱导的circMAN1A2对宫颈癌细胞恶性行为的影响。方法:RNA高通量测序检测TNF-α处理后人宫颈癌HeLa细胞中circRNA的表达差异性。RT-qPCR方法验证TNF-α对circMAN1A2的影响。RNase R耐受性实验验证circMAN1A2是否耐受RNase R消化。核质RNA分离实验研究circMAN1A2在HeLa细胞的定位;Transwell迁移侵袭实验、MTT实验、集落形成实验研究circMAN1A2对宫颈癌细胞恶性行为的影响。结果:RNA高通量测序检测到98种表达上调和63种表达下调的circRNA。TNF-α处理后circMAN1A2表达上调,其能够耐受RNase R消化,主要定位于HeLa细胞胞核中。细胞功能实验表明circMAN1A2能够促进宫颈癌细胞的迁移、侵袭和增殖。结论:TNF-α诱导的circMAN1A2促进宫颈癌细胞的迁移、侵袭和增殖。

Objective:To identify the effect of tumor necrosis factor(TNF)-α-induced circMAN1A2 on the malignant behavior of cervical cancer cells.Methods:RNA high-throughput sequencing was used to detect the difference of circRNA expression in human cervical cancer HeLa cells treated with TNF-α.RT-qPCR was used to verify the effect of TNF-α on circMAN1A2.The RNase R resistance assay verified whether circMAN1A2 was resistant to RNase R digestion.RNA isolation experiment was performed to investigate the localization of circMAN1A2 in HeLa cells.Transwell migration/invasion assay,MTT assay,and colony formation assay were used to study the effects of circMAN1A2 on the malignant behavior of cervical cancer cells.Results:RNA high-throughput sequencing found 98 up-regulated and 63 down-regulated circRNA.circMAN1A2 was up-regulated after TNF-α treatment,which was resistant to RNase R digestion and was mainly localized in the nuclear of HeLa cells.Cell function experiments showed that circMAN1A2 could promote the migration,invasion and proliferation of cervical cancer cells.Conclusion:TNF-α-induced circMAN1A2 promotes the migration,invasion and proliferation of cervical cancer cells.