乳腺癌中组蛋白去甲基化酶JMJD3与上皮间质转化相关蛋白表达的相关性及临床意义

Correlation of JMJD3 and Epithelial-mesenchymal Transition-related Protein Expression in Breast Cancer and Clinical Significance

目的探讨乳腺癌组织中组蛋白去甲基化酶JMJD3与上皮细胞-间质转化(EMT)相关蛋白E-cadherin、Vimentin和N-cadherin的表达及其相关性。方法收集120例乳腺癌及对应癌旁组织标本,采用免疫组织化学SP法检测乳腺癌组织中JMJD3、E-cadherin、Vimentin和N-cadherin的表达,并分析上述蛋白与乳腺癌临床病理特征的关系及相关性。结果乳腺癌组织中JMJD3和E-cadherin阳性率(56.67%和65.00%)显著低于癌旁组织(73.33%和83.33%)(均P<0.05),Vimentin和N-cadherin阳性率(54.17%和58.33%)显著高于癌旁组织(23.33%和20.83%)(均P<0.05)。JMJD3、E-cadherin、Vimentin及N-cadherin的表达与乳腺癌组织学分级、TNM分期和淋巴结转移均相关(均P<0.05),JMJD3与肿瘤直径相关(P<0.05)。Spearman相关分析结果显示,乳腺癌中JMJD3与E-cadherin表达呈正相关(r=0.204,P=0.025),与Vimentin和N-cadherin表达均呈负相关(r=-0.467,r=-0.500,均P=0.000)。结论乳腺癌中JMJD3可能通过调节乳腺癌细胞上皮-间质转化途径调控乳腺癌侵袭转移。

Objective To investigate the expression of histone demethylase JMJD3 and epithelialmesenchymal transition(EMT)related proteins in breast cancer and their correlation.Methods Immunohistochemical SP method was used to detect the expression of JMJD3 and EMT-related proteins E-cadherin,Vimentin and N-cadherin in 120 cases of breast cancer and adjacent tissues.The correlation of JMJD3,EMT-related proteins with clinicopathological characteristics of breast cancer patients was analyzed.Results The positive rates of JMJD3 and E-cadherin in breast cancer tissues(56.67%and 65.00%)were significantly lower than those in adjacent tissues(73.33%and 83.33%)(P<0.05).The positive rates of Vimentin and N-cadherin in breast cancer tissues(54.17%and 58.33%)were significantly higher than those in adjacent tissues(23.33%and 20.83%)(P<0.05).The expression of JMJD3,E-cadherin Vimentin and N-cadherin were correlated with histological classification,TNM stage and lymph node metastasis of breast cancer.JMJD3 was correlated with tumor diameter(P<0.05).Spearman correlation analysis showed that JMJD3 expression was positively correlated with E-cadherin expression in breast cancer(r=0.204,P=0.025),while JMJD3 was negatively correlated with Vimentin and N-cadherin expression(r=-0.467,r=-0.500,P=0.000).Conclusion JMJD3 may regulate the invasion and metastasis of breast cancer through regulating EMT.