黄花败酱化学成分研究

Study on chemical constituents of Patrinia scabiosaefolia

对黄花败酱Patrinia scabiosaefolia的化学成分进行研究。采用硅胶、Sephadex-LH 20、制备等多种色谱分离技术进行提取分离纯化,然后应用波谱技术进行结构鉴定,从黄花败酱全草95%乙醇提取物中共分离得到24个化合物,分别鉴定为:8,9-didehydro-7-hydroxydolichodial(1)、convoldorine(2)、3-O-p-香豆酰奎尼酸(3)、松柏醛(4)、芥子醛(5)、C-藜芦酰乙二醇(6)、2,3-dihydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone(7)、莨菪亭(8)、异莨菪苷(9)、七叶苷(10)、clovin(11)、robinin(12)、苄基葡萄糖苷(13)、淫羊藿次苷F2(14)、2-苯乙基-1-O-β-D-葡萄糖苷(15)、淫羊藿次苷D1(16)、大黄素(17)、β-谷甾醇(18)、熊果酸(19)、2α,6α-dihydroxy-5-[(E)-3′-hydroxy-3′-methyl-1′-butenyl]-6-methyl-4-cyclohexen-3-one(20)、loliolide黑麦草内酯(21)、isololiolide异黑麦草内酯(22)、3,4,5-三甲氧基苯酚-l-O-β-D-葡萄糖苷(23)、对羟基苯甲醛(24)。其中,化合物1为新天然产物,化合物2、3、5~7、9~17、20、22~24为首次从败酱属中分离得到。以上化合物对人结肠癌细胞HCT-116均未表现出抑制活性。

To study the chemical constituents of Patrinia scabiosaefolia,chromatography,such as silica gel,Sephadex-LH 20,Pre-HPLC,were used for separation and purification,then the structure was identified by spectral technique.Twenty-four compounds were isolated and determined as 8,9-didehydro-7-hydroxydolichodial(1),convoldorine(2),3-O-p-coumaroylquinic acid(3),coniferaldehyde(4),sinapaldehyde(5),C-veratroylglycol(6),2,3-dihydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone(7),scopoletin(8),7-methoxycoumarin-6-β-D-glucopyranoside(9),aseculin(10),clovin(11),robinin(12),benzyl-O-β-D-glucopyranoside(13),icariside F2(14),2-phenylethyl-1-O-β-D-glucopyranoside(15),icariside D1(16),emodin(17),β-sitosterol(18),ursolic acid(19),2α,6α-dihydroxy-5-[(E)-3′-hydroxy-3′-methyl-1′-butenyl]-6-methyl-4-cyclohexen-3-one(20),loliolide(21),isololiolide(22),3,4,5-trimethoxyphenyl-1-β-D-glucopyranoside(23),p-hydroxybenzaldehyde(24).Compound 1 is a new natural compound,and compounds 2,3,5-7,9-17,20,22-24 were isolated from this genus for the first time.The screening experiment of cytotoxic activity indicated that all the compounds don′t show inhibition on human colon cancer HCT-116 cells.