阿胶糕中脂肪含量测定方法研究

Study on the Determining Method for the Fat Content in Donkey-hide Gelatin (Ejiao) Cake

阿胶糕因含有阿胶、糖类等物质,产品的韧性和黏度比较大,GB 5009.6-2016标准规定的样品制备方法无法将阿胶糕制备成均匀一致的样品。现有标准中的脂肪提取方法无法满足阿胶糕脂肪提取的要求,酸水解提取过程中炭化反应会导致脂肪测定结果偏高,索氏抽提方法加热提取时阿胶和糖类物质的黏度增大,会将核桃仁和芝麻等包裹,样品中的脂肪无法得到有效的提取。针对阿胶糕产品特性,将样品-18 ℃冷冻4 h以上后进行粉碎,制备的样品可以满足检验要求,对制备的样品经超声处理后直接使用有机溶剂提取,经验证结果的准确性和精密性比较好。该方法的通用性和可推广性比较好,能满足产品质量控制的要求。

Because of containing donkey-hide gelatin,sugar and so on,the toughness and viscosity of the product are relatively big,the sample preparation method stipulated in GB 5009.6-2016 standard can not make the donkey-hide gelatin cake into uniform sample.The existing standard methods of fat extraction can not meet the requirements of fat extraction of donkey-hide gelatin (Ejiao) cake,the carbonization reaction in the process of acid hydrolysis extraction will lead to higher results of fat determination,the viscosity of donkey-hide gelatin and carbohydrate substances will increase when sample is heated to extract by the soxhlet extraction method,and the walnut kernel and sesame seeds will be wrapped up,the fat in the sample can not be extracted effectively.According to the characteristics of donkey-hide gelatin (Ejiao) cake,the samples were frozen at-18 ℃ for more than four hours and crushed.The prepared samples could meet the inspection requirements.The samples were extracted directly by organic solvent after ultrasonic treatment,and the accuracy and precision of the results were proved to be better.The generality and popularization of the method were better.To meet the requirements of product quality control.