金属-有机框架材料在活性肽富集中的应用

Application of Metal-Organic Framework Materials in Enrichment of Active Peptides

生物活性肽在整个生理系统当中发挥着重要作用,对于生物活性肽的精确分析将有助于进一步开发其功效,然而当前对复杂生物系统中肽的分析依然存在相当大的难度,这是由于肽通常与高浓度蛋白质共存这一特质所造成的,严重降低了色谱中肽的分离效率,并在质谱中抑制肽的峰信号。鉴于此,人们引入金属-有机框架材料对活性肽进行富集分析。金属-有机框架(MOFs),是由金属离子或团簇和有机配体,通过配位键自行组装形成的具有多孔结构的有机-无机杂化材料。由于它们具有框架结构可调、高孔隙率、化学稳定性良好、可再生性、合成过程简单等优点而广泛应用于活性肽富集、气体吸附与分离、传感器、药物缓释与催化反应等领域。本文系统梳理了近年来MOFs材料用于磷酸肽、糖肽和内源肽等活性肽富集的研究进展,在此基础上总结了当前MOFs材料在该领域中存在的局限,并对研究新趋向提出了展望。

Bioactive peptides play an important role in physiological processes.Accurate analysis of bioactive peptides will help to further develop their efficacy.Due to the bioactive peptides often coexisting with high concentrations of proteins,it is still quite difficult to analyze peptides in complex biological systems.These coexisted proteins may severely reduce the separation efficiency of chromatographic column for bioactive peptides and inhibit the peak signal of the peptide in the mass spectrum.In view of this,metal-organic frameworks are introduced to perform enrichment analysis of active peptides.Metal-organic frameworks(MOFs)is a type of framework self-assembled by coordination bonds between metal ions or metal clusters and organic ligands to form organic-inorganic hybrid materials with porous structure.Due to their adjustable frame structure,high porosity,good chemical stability,reproducibility,and simple synthesis process,they are widely used in active peptides enrichment,gas adsorption and separation,the areas of sensors,drug delivery and catalytic reactions.In this paper,the research progress of MOF materials as active peptide enrichment materials such as phosphopeptides,glycopeptides and endogenous peptides in recent years is reviewed,and the shortcomings and outlook of MOFs materials in this area are pointed out.