CRIM1基因对胃癌细胞增殖和凋亡的影响及其预后价值

Effects of CRIM1 on the proliferation and apoptosis of gastric cancer cells and the prognostic value of CRIM1 expression

目的探讨富含半胱氨酸型运动神经元蛋白1(CRIM1)在胃癌中的预后价值,并研究沉默CRIM1后对胃癌细胞增殖和凋亡的影响。方法应用TCGA和Kaplan-Meier plotter数据库分别分析CRIM1在胃癌中的表达情况及其与胃癌患者预后的关系。通过转染小干扰RNA(siRNA)降低CRIM1在AGS细胞中的表达并验证沉默效果。采用MTT法和流式细胞术分别验证CRIM1沉默对AGS细胞增殖和凋亡的影响。Western blotting检测AGS细胞中凋亡相关蛋白BCL-2和BAX的表达。结果生物信息学分析结果显示,胃癌组织中CRIM1的表达明显高于正常胃组织,且CRIM1低表达的胃癌患者比高表达的胃癌患者总生存期更长(P<0.05)。转染组AGS细胞的CRIM1蛋白表达量显著低于空白对照组和阴性对照组(P<0.05)。沉默CRIM1后AGS细胞的增殖率降低,凋亡率升高,BCL-2蛋白的表达量下降而BAX蛋白的表达量升高(P<0.05)。结论高表达的CRIM1与胃癌患者预后不良有关,沉默CRIM1可以抑制胃癌细胞AGS增殖,并诱导其凋亡。

Objective To investigate the prognostic value of cysteine-rich motor neuron 1(CRIM1)expression in gastric cancer(GC)and the effects of silencing CRIM1 on the proliferation and apoptosis of GC cells.Methods TCGA and Kaplan-Meier plotter databases were used to analyze CRIM1 expression in GC and its relationship with the prognosis of GC patients.The expression of CRIM1 in AGS cells was reduced by siRNA transfection,and the effect of silencing was verified.MTT assay and flow cytometry were performed to investigate the effects of silencing CRIM1 on the proliferation and apoptosis of AGS cells.Western blotting was used to detect the expression of the apoptosis-related proteins BCL-2 and BAX in AGS cells.Results The results of bioinformatics analysis showed that CRIM1 expression in GC tissues was significantly higher than that in normal gastric tissues and that GC patients with low CRIM1 expression had longer overall survival than those with high CRIM1 expression(P<0.05).The expression of CRIM1 protein in the group of transfected AGS cells was significantly lower than that in the blank control group and the negative control group(P<0.05).In CRIM1-silenced AGC cells relative to control cells,the proliferation rate was decreased,the apoptosis rate was increased,the expression of BCL-2 protein was decreased,and the expression of BAX protein was increased(P<0.05).Conclusion High expression of CRIM1 is associated with poor prognosis of GC patients.Silencing CRIM1 can inhibit the proliferation of AGS cells and induce their apoptosis.