基于生物信息学分析F13A1基因及蛋白质

Analysis of F13A1 Gene and Protein Based on Bioinformatics

目的利用生物信息学方法对F13A1及其编码蛋白质进行分析,为F13A1的实验研究提供理论基础和新线索。方法从UCSC(http://genome.ucsc.edu/index.html)数据库检索“F13A1”,下载F13A1蛋白序列,截取转录起始点上游2000 bp碱基序列作为启动子区域进行分析,分析F13A1编码蛋白的理化性质、亚细胞定位、信号肽和跨膜区域、蛋白质结构、相互作用网络及GO注释。结果F13A1包含多个启动区域,不存在CpG岛,但存在SRF、Sp1和AP-1等转录因子结合位点。F13A1编码蛋白是由732个氨基酸组成的无信号肽、无跨膜区域的亲水蛋白,其主要定位于细胞核,二级结构以随机卷曲为主。另外蛋白F13B、FGA、FGG、F2、FGB、FN1、SERPINF2、VWF、VEGFA、F5可能与F13A1存在相互作用。结论F13A1基因存在多个启动子区域,拥有多个转录因子结合位点;F13A1蛋白与F13B、FGA、FGG、F2等多个蛋白质相互作用,其参与组成内质网腔、血小板α颗粒内腔以及细胞内细胞器腔等多种细胞成分,同时在血液凝固、纤维蛋白溶解和血小板活化等多种生理过程发挥重要作用。

Objective To analyze F13A1 and its encoded protein using bioinformatics methods,and provide theoretical basis and new clues for the experimental study of F13A1.Methods Search"F13A1"from the UCSC(http://genome.ucsc.edu/index.html)database,download the F13A1 protein sequence,and intercept the 2000 bp base sequence upstream of the transcription start point as a promoter region for analysis,the physicochemical properties,subcellular localization,signal peptide and transmembrane region,protein structure,interaction network and GO annotation of F13A1 encoded protein were analyzed.Results F13A1 contains multiple promoter regions,there is no CpG island,but there are transcription factor binding sites such as SRF,Sp1 and AP-1.The protein encoded by F13A1 is a hydrophilic protein composed of 732 amino acids with no signal peptide and no transmembrane region.It is mainly located in the nucleus,and the secondary structure is dominated by random coils.In addition,proteins F13B,FGA,FGG,F2,FGB,FN1,SERPINF2,VWF,VEGFA,F5 may interact with F13A1.Conclusion The F13A1 gene has multiple promoter regions with multiple transcription factor binding sites;the F13A1 protein interacts with multiple proteins such as F13B,FGA,FGG,and F2,and is involved in the formation of the endoplasmic reticulum cavity,plateletαparticle cavity,and cells Various cellular components such as the inner organelle cavity play an important role in various physiological processes such as blood coagulation,fibrinolysis and platelet activation.