QuEChERS-高效液相色谱法检测油料作物中氟磺胺草醚残留量

Determination of fomesafen residue in oil crops by QuEChERS-high performance liquid chromatography

目的建立QuEChERS-高效液相色谱联用技术检测油菜籽、大豆、花生中氟磺胺草醚残留量的方法。方法样品用1%甲酸水溶液和乙腈提取,经N-丙基乙二胺粉末净化,色谱柱分离,以0.1%甲酸水溶液和乙腈为流动相进行洗脱,二极管阵列检测器检测,检测波长295 nm。结合保留时间和紫外光谱进行定性分析,峰面积进行定量分析。结果氟磺胺草醚在0.5~100μg/mL范围内线性良好,相关系数(r^2)为0.9999,方法检出限为0.021 mg/kg;在油菜籽、大豆、花生中3个添加水平的平均回收率为89.9%~102.3%,相对标准偏差为2.5%~7.2%。结论该方法简便、快捷、节约溶剂,适用于油料作物中检测氟磺胺草醚残留量。

Objective To establish a method for the determination of fomesafen residue in rapeseed,soybean and peanut by using QuEChERS with high performance liquid chromatography.Methods Thesamples were extracted by 1%formic acid and acetonitrile,purified by N-(n-Propyl)ethylenediamine powder,separated by chromatographic column,eluted by 0.1%formic acid and acetonitrile,and detected by diode array detectorwith the detection wavelength 295 nm.The targets were determined by retention time and ultraviolet spectrum,and quantified by peak area.Results Fomesafen had a good linearity in the range of 0.5 to 100μg/mL,the correlation coefficient(r^2)was 0.9999,and the limit of detection was 0.021 mg/kg.The average recoveries of the 3 added levels in rapeseed,soybean,and peanut were 89.9%–102.3%,and the relative standard deviations were 2.5%–7.2%.Conclusion This method is easy,fast,solvent-saving,thus it is suitable for detection of fomesafen in oil crops.