分光光度法测定桑黄中总黄酮的不确定度评定

Evaluation of uncertainty in determination of total flavone in Phellinus spp.by spectrophotometer

目的评定分光光度法测定桑黄中总黄铜的不确定度。方法依据JJF1059.1-2012《测量不确定度评定与表示》的方法,建立相应的数学模型。对测量过程中可能引入不确定度的来源进行分析与计算,进而合成出相对不确定度和扩展不确定度。结果标准曲线是不确定度主要来源,其中曲线拟合所占比例最大。结论在总黄酮测定过程中,要注意选择线性好的标准曲线。

Objective To evaluate the uncertainty of determination of caffeine in total brass using phellinus linteus spectrophotometer.Methods According to JJF1059.1-2012 evaluation and Expression of Uncertainty in Measurement,a mathematical model was established to determine the uncertainty of total brass in phellinus linteus spectrophotometer.The sources of uncertainty which may be introduced into the measurement process were analyzed and calculated,and then the relative uncertainty and extended uncertainty were synthesized.Results The standard curve was a major source of uncertainty,curve fitting account for the largest proportion.Conclusion In the determination process of total flavonoids,care should be taken to select a good linear standard curve.